QUANTITATIVE ESTIMATION OF URINE SEDIMENT IN HEALTHY WISTAR RATS

DOI: 10.29296/2618723X-2022-01-01

A.Yu. Kirsanova, N.V. Kubrak

Federal State Budgetary Institution «National Ilizarov Medical Research Centre for Traumatology and Ortopaedics» Ministry Healthcare

640014, Russian Federation, Kurgan, M. Ulyanova st., 6

Е-mail: [email protected]


Keywords: urine sediment rat norm reference range

For citation:

Kirsanova A.Yu., Kubrak N.V. QUANTITATIVE ESTIMATION OF URINE SEDIMENT IN HEALTHY WISTAR RATS. Laboratory Animals for Science. 2022; 1. https://doi.org/10.29296/2618723X-2022-01-01

Abstract

No biological experiment is carried out without the participation of laboratory animals, while rats are most often used, which are a stable model that allows you to reproduce various pathological processes. For obtaining accurate data and their correct interpretation, it is important that the state of the experimental animals at the starting point of the experiment is as similar as possible.  At the same time, the study of urine sediment is the easiest way to obtain information about the state of the organism. To diagnose a pathological process, it is necessary to compare results of examination with data characterizing the state of health within the same biological species. However, there is not enough scientific information about urine sediment in healthy rats, so, in many studies there is a tendency to compare the data obtained in an experiment with the participation of laboratory animals with data determined for a healthy human.  The work presents the results of a study of urine sediment obtained from 100 clinically healthy Wistar rats (52 females and 48 males), aged 6–12 months, weighing 250–350 g. The urine sediment of the morning portion of the urine of animals, after centrifugation and preparation, was examined using a light microscope. All actions for the preparation of native samples were performed in accordance with a specific algorithm, under equal conditions. The elements of the sediment were assessed according to common criteria for their identification, the quantitative value of various elements of the urine sediment was determined, followed by the calculation of the reference intervals for each element.

Full text avaliable in Russain only 

Acknowledgments

The authors declare no sponsorship funding for the research.

Contribution of authors

Kirsanova A.Yu. – idea, analysis and interpretation of results, statistical data processing, writing and editing of text.

Kubrak N.V. – collection and systematization of material, text editing, approval of the final version of the article.

 

Conflict of interest

The authors declare no conflict of interest.

References

  1. Проблема нормы в токсикологии / под ред. И.М. Трахтенберга. – М : Медицина, 1991. - 204 с. [Problema normy v toksikologii / pod red. I.M. Trakhtenberga. – M : Meditsina, 1991. - 204 p. (In Russ)].
  2. Краснов В.В., Кубрак Н.В., Кирсанова А.Ю. Развитие экспериментальной вертебрологии и нейрохирургии в Российском научном центре "Восстановительная травматология и ортопедия" им. академика Г.А. Илизарова // Гений ортопедии. - 2017. - №23(2). - С. 134-139. [Krasnov V.V., Kubrak N.V., Kirsanova A.Yu. Razvitie eksperimental'noi vertebrologii i neirokhirurgii v Rossiiskom nauchnom tsentre "Vosstanovitel'naya travmatologiya i ortopediya" im. akademika G.A. Ilizarova // Genii ortopedii. - 2017. - №23(2). - P. 134-139. (In Russ)]. DOI:10.18019/1028-4427-2017-23-2-134-139
  3. Соболев В.Е., Клинический и биохимический анализ мочи крыс при экспериментальном геморрагическом цистите // Ученые записки орловского государственного университета. - №7(63). - 2014. - С. 257-258. [Sobolev V.E., Klinicheskii i biokhimicheskii analiz mochi krys pri eksperimental'nom gemorragicheskom tsistite // Uchenye zapiski orlovskogo gosudarstvennogo universiteta. - №7(63). - 2014. - P. 257-258 (In Russ)].
  4. Стогов М.В., Овчинников Е.Н. Лабораторные тесты в доклинической диагностике остеоартроза. Аналитический обзор // Гений Ортопедии. - 2016. -1. - С. 96-103. [Stogov M.V., Ovchinnikov E.N. Laboratornye testy v doklinicheskoi diagnostike osteoartroza. Analiticheskii obzor // Genii Ortopedii. - 2016. - 1. - P. 96-103 (In Russ)]. DOI:10.18019/1028-4427-2016-1-96-103
  5. Кирсанова А.Ю., Кубрак Н.В. Сравнительное исследование вариантов медикаментозной терапии в профилактике осложнений контузионной травмы спинного мозга на основании анализа состояния мочевыделительной системы // Современные проблемы науки и образования. - 2021. - 3. [Kirsanova A.Yu., Kubrak N.V. Sravnitel'noe issledovanie variantov medikamentoznoi terapii v profilaktike oslozhnenii kontuzionnoi travmy spinnogo mozga na osnovanii analiza sostoyaniya mochevydelitel'noi sistemy // Sovremennye problemy nauki i obrazovaniya. - 2021. - 3 (In Russ)]. DOI:10.17513/spno.30932
  6. Справочник. Физиологические, биохимические и биометрические показатели нормы экспериментальных животных / под ред. Макарова В.Г. - СПб: Лема. - 2013. - 118 с. [Spravochnik. Fiziologicheskie, biokhimicheskie i biometricheskie pokazateli normy eksperimental'nykh zhivotnykh / pod red. Makarova V.G. - SPb: Lema. - 2013. - 118 p (In Russ)].
    Sharp P., Villano J. S. The laboratory rat / CRC press. - 2012.
  7. Трофимец Е.И., Кательникова А.Е., Крышень К.Л. Обзор по получению образцов мочи у лабораторных животных // Лабораторные животные для научных исследований. - 2021. - 1. C. 30-47. [Trofimets E.I., Katel'nikova A.E., Kryshen' K.L. Obzor po polucheniyu obraztsov mochi u laboratornykh zhivotnykh // Laboratornye zhivotnye dlya nauchnykh issledovanii. - 2021. - 1. P. 30-47 (In Russ)]. https://doi.org/10.29296/2618723X-2021-01-04
  8. Миронова И.И., Романова Л.А. Атлас осадков мочи / Тверь: Триада. – 2003. - 139 с. [Mironova I.I., Romanova L.A. Atlas osadkov mochi / Tver': Triada. - 2003. 139 p (In Russ)].
  9. Юнкеров В.И., Григорьев С.Г. Математико-статистическая обработка данных медицинских иследований / Спб : ВМедА - 2005. - 266 с. [YUnkerov V.I., Grigor'ev S.G. Matematiko-statisticheskaya obrabotka dannyh medicinskih isledovanij / Spb : VMedA - 2005. - 266 p (In Russ)].

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