Comparative evaluation of various methods of experimental modeling of neuropathic pain syndrome in rats

N.V. Aleksandrovskaya, PhD,
A.A. Kruglova, researcher (responsible for the correspondence)

Federal State Unitary Enterprise "Scientific centre "Signal",
Russian Federation, 107014, Moscow, St. Bolshaya Olenya, 8


Pain results from the activation of multiple sensory neurons called nociceptors, it has evolved as a "detection and defense" mechanism. However, damage or disease of the sensory system can lead to neuropathic pain, which has no protective function. Neuropathic pain syndrome (NBS) is a widespread disease that significantly worsens the life quality. The reasons for NBS can be: compression (compression), inflammation, intoxication, violation of blood supply and nutrition (ischemia) of nerve tissues. Manifestations of neuropathy can be very diverse, depending on the localization of the process: facial nerve neuropathy, ulnar nerve neuropathy, trigeminal nerve neuropathy, radial nerve neuropathy, sciatic nerve neuropathy, etc.; on the functions of the nerve – motor, sensory or vegetative. One of the largest in humans and animals is the sciatic nerve (N. ischiadicus), it is a continuation of the anterior branches of the LIV–SIII spinal nerves. Sciatic nerve neuropathy is characterized by symptoms such as numbness, pain in the hip and lower leg, sagging feet when walking, the inability to normally lean on the foot, as well as loss of function of the peroneal and tibial nerves (hypersensitivity to external stimuli (hyperesthesia), anesthesia of the entire lower leg and foot, paralysis of the foot and fingers, loss of the Achilles reflex, dry skin or hyperhidrosis, impaired coordination of movements, etc.). When developing drugs for the treatment of NBS of different etiologies, a model is needed that most accurately reflects the development and symptoms of a real disease. The aim of this work was a comparative evaluation of the effectiveness of modeling of neuropathic pain syndrome created by different types of effects on the sciatic nerve. During the experiment, among the investigated mechanical methods of effects on the sciatic nerve, the most effective for modeling NBS, were ligation of the nerve trunk and the imposition of a metal clamp. Among the studied chemical methods of effects on the sciatic nerve, the most effective for modeling NBS can be considered an injection of 37% hydrogen peroxide.

Full text avaliable in Russain only 

Authors contribution

Alexandrovskaya N.V. – a significant contribution to the concept of work, writing an article for publication, approval of the final version of the article for publication, consent to be responsible for all aspects of the work;

Kruglova A.A. – development of the design of the method, experimental work, collection and analysis of data, work with literary sources, participation in writing an article for publication, preparation of photo materials for the article.


The work was done without sponsorship.



Conflict of interest

The authors declare that there is no conflict of interest.


  1. Smith E.S.J. Advances in understanding nociception and neuropathic pain // J Neurol. – 2018. – Vol. 265 (2). – P. 231-238. DOI: 10.1007/s00415-017-8641-6.
  2. Dubin A.E., Patapoutian A. Nociceptors: the sensors of the pain pathway // J Clin Investig. – 2010. – Vol. 120. – P. 3760-3772. doi: 10.1172/JCI42843.
  3. Baheti D.K. Neuropathic pain-recent trends in management // Pain Management Clinic, Bombay Hospital Institute of Medical Sciences, Mumbai. PMID: 12022219
  4. Barbosa A.B.M., Santos P.V.D., Targino V.A., Silva N.A., Silva Y.C.M., Gomes FB, Assis TO. Sciatic nerve and its variations: is it possible to associate them with piriformis syndrome? // Arq Neuropsiquiatr. – 2019. – Vol. 23 №77(9). – P. 646-653. doi: 10.1590/0004-282X20190093. eCollection 2019.
  5. Нервные болезни: учебник для студентов медицинских вузов / под ред. М. М. Одинака. – СПб: СпецЛит, 2014. – 526 с. [Nervnye bolezni: uchebnik dlya studentov meditsinskikh vuzov / pod red. M. M. Odinaka. – SPb: SpeTSLit, 2014. – 526 p. (In Russ.)].
  6. Анатомия крысы (Лабораторные животные) / Под ред. Академика А. Д. Ноздрачева. – СПб.: Изд-во «Лань», 2001. – 464 с. [Anatomiya krysy (Laboratornye zhivotnye) / Pod red. Akademika A. D. Nozdracheva. – SPb.: Izd-vo «Lan'», 2001. – 464 p. (In Russ.)].
  7. Burma NE, Leduc-Pessah H, Fan CY, Trang T. Animal models of chronic pain: advances and challenges for clinical translation: animal models of chronic pain // J Neurosci Res. – 2017. – Vol. 95. – P. 1242-1256. doi: 10.1002/jnr.23768.
  8. Miller J.J., Aoki K., Moehring F., Murphy C.A., O'Hara C.L., Tiemeyer M., Stucky C.L., Dahms N.M. Neuropathic pain in a Fabry disease rat model // JCI Insight. – 2018. – Vol. 22 №3(6). – P. 1-19. doi: 10.1172/jci.insight.99171. PMID: 29563343
  9. Абдулкина Н.Г., Левицкий Е.Ф., Кочегуров B.А. Алгоритмизация физиотерапии травм периферических нервов. – Томск: Изд-во «Печатная мануфактура», 2007. – 248 с. [Abdulkina N. G., Levitskii E. F., Kochegurov B. A. Algoritmizatsiya fizioterapii travm perifericheskikh nervov. – Tomsk: Izd-vo «Pechatnaya manufakturA», 2007. – 248 p. (In Russ.)].
  10. Руководство по проведению доклинических исследований лекарственных средств. Часть первая / Под ред. А.Н. Миронова. – М.: Гриф и К, 2012. – 944 с. [Rukovodstvo po provedeniyu doklinicheskikh issledovanii lekarstvennykh sredstv. Chast' pervaya / Pod red. A.N. Mironova. – M.: Grif i K, 2012. – 944 s.]
  11. Картищенко Н. Н. Основы биомоделирования. – М.: Изд-во ВПК, 2004. – 608 с. [Kartishchenko N. N. Osnovy biomodelirovaniya. – M.: Izd-vo VPK, 2004. – 608 s. (In Russ.)].
  12. Крупаткин А.И. Клиническая нейроангиофизиология конечностей (периваскулярная иннервация и нервная трофика). – М.: Научный мир, 2003. – 328 с. [Krupatkin A. I. Klinicheskaya neiroangiofiziologiya konechnostei (perivaskulyarnaya innervatsiya i nervnaya trofika). – M.: Nauchnyi mir, 2003. – 328 p. (In Russ.)].

You may be interested