Activation Response Of Testicular Endocrine System Induced By The Exposure To A Female In C57BL/6J Mice Activation Response Of Testicular Endocrine System Induced By The Exposure To A Female In C57Bl/6J Mice

E. T. Amstislavskaya(1,2), Doctor of biological sciences, Deputy Director for Science, Chief Scientist, Head of laboratory of translational biopsychiatry; S. N. Novikov(3), doctor of biological Sciences, expert 1-Federal State Budgetary Scientific Institution “Scientific Research Institute of Physiology and Basic Medicine” (SRIPhBM), 630117, Timakova Str., 4., Novosibirsk, Russia. 2-Novosibirsk State University, 630090, Pirogova Str., 1., Novosibirsk, Russia. 3-Scientific Research Institute – Federal Research Centre for Projects Evaluation and Consulting Services, 123317, Antonova-Ovseenko Str. 13, Bldg. 1, Moscow, Russia. Е-mail: amstislavskayatg@physiol.ru

Abstract

C57BL/6J mice are widely used in various medical and biological studies. However, characteristic increase of sex hormones in the males’ blood in response to a sexual stimulus was not detected. In the present work, we studied the 60-minute dynamics of behavior of male C57BL/6J and testosterone level in the blood under conditions that allow it to see and smell, but not allowing direct contact with receptive female. The study revealed a significant increase in testosterone of the male blood of C57BL/6J by the 30th minute in comparison with its initial values, followed by its decrease. During the first 30 minutes from the moment of appearance of the receptive female, the presence of the pronounced sexual motivational males’ behavior, which preceded the increase of the level of testosterone in the blood, was recorded. After 30 minutes its rapid decrease was noted. Sexual motivational behavior of males was assessed by the time of the male’s occupation at the holed partition separating the female. Thus, for the first time, the activating effect of the female on testosterone levels in the peripheral blood of male C57BL/6J mice, accompanied by pronounced sexual motivation, was recorded. The work clearly demonstrated that during sexual stimulation the male C57BL/6J activation of the hypothalamic-pituitary-testicular complex occur. The rise of the testosterone level, caused by the presence of the receptive female, can be considered a reliable parameter characterizing the sexual stimulation of the male.

References

  1. Amstislavskaya T.G., Hrapova M.V. Vliyanie genotipa na povedencheskuyu i gormonal`nuyu komponenty polovoy aktivacii samcov myshey. Byull. e`ksper. biol. med. 2002. T. 133, 5: 548–51.
  2. Novikov S.N. Feromony i razmnozhenie mlekopitayushhih. M.: Nauka (LO). – 1988; 168 s.
  3. Novikov S.N., Churakov G.A., Filimonenko A.A., Ermakova I.I., Fedorova E.M., Burkot I.A. Vliyanie genotipa i pola na e`kspressiyu belkov mochi kompleksa MUP v postnatal`nom ontogeneze myshi. Ontogenez. 2009; 40 (4): 261–9.
  4. Novikov S.N., Troickaya V.T., Gladysheva O.S., Churakov G.A. Specificheskaya anosmiya k izovalerianovoy kislote u laboratornyh myshey linii C57BL/6. Dokl. RAN. 2002; 387 (4): 562–4.
  5. Osadchuk A.V., Naumenko E.V. Rol` genotipa i nekotoryh vidov zoosocial`nogo povedeniya v regulyacii e`ndokrinnoy funkcii semennikov u myshey. Dokl. AN SSSR. 1981; 258 (3): 746–9.
  6. Popova N.K., Amstislavskaya T.G., Kucheryavyy S.A. Polovaya motivaciya samcov myshey, inducirovannaya prisutstviem samki. Zhurn. vyssh. nervn. deyat. 1998; 46 (1): 84–90.
  7. Amstislavskaya T.G., Popova N.K. Female-induced sexual arousal in male mice and rats: behavioral and testosterone response. Horm. Behav., 2004; 46 (5): 544–50.
  8. Amstislavsky S.Ya., Amstislavskaya T.G., Amstislavsky V.S., Tibeikina M.A., Osipov K.V., Eroschenko V.P. Reproductive abnormalities in adult male mice following preimplantation exposures to estradiol or pesticide methoxychlor. Reprod. Toxicol. 2006; 21 (2): 154–9.
  9. Bacchini A., Gaetani E., Cavaggioni A. Pheromone binding proteins of the mouse, Mus musculus. Experientia. 1992. 48 (4): 419–921.
  10. Cheetham S.A., Smith A.L., Armstrong S.D., Beynon R.J., Hurst J.L. Limited variation of the major urinary proteins of laboratory mice. Physiol. Behav. 2009; 96 (2): 253–61. DOI: 10.1016/j.physbeh. 2008.10,005
  11. Churakov G., Philimonenko A.A., Novikov S. Genetic control and expression of the major urinary proteins (MUPs) in laboratory mice: MUPs as potential pheromone messengers? Chem. Senses. 1992; 17: 604.
  12. Johnston R.E. Pheromones, the vomeronasal system, and communication. From hormonal responses to individual recognition. Ann. NY Acad. Sci. 1998; 855: 333–48.
  13. Griff I.C., Reed R.R. The genetic basis for specific anosmia to isovaleric acid in the mouse. Cell. 1995; 83 (3): 407–14.
  14. Kelliher K.R. The combined role of the main olfactory and vomeronasal systems in social communication in mammals. Horm. Behav. 2007; 52 (5): 561–70. DOI: 10.1016/j.yhbeh.2007.08.012.
  15. Kwak J., Grigsby C.C., Rizki M.M., Preti G., Köksal M., Josue J., Yamazaki K., Beauchamp G.K. 2012. Differential binding between volatile ligands and major urinary proteins due to genetic variation in mice. Physiol. Behav., 2012, Vol. 107 (1): 112–20. DOI: 10.1016/j.physbeh.2012.06.008.
  16. Mucignat-Caretta C., Redaelli M., Orsetti A., Perriat-Sanguinet M., Zagotto G., Ganem G. Urinary volatile molecules vary in males of the 2 European subspecies of the house mouse and their hybrids. Chem. Senses. 2010; 35 (8): 647–54. DOI: 10.1093/chem.se/bjq049.
  17. Nyby J.G. Reflexive testosterone release: a model system for studying the nongenomic effects of testosterone upon male behavior. Front Neuroendocrinol. 2008; 29 (2): 199–210. DOI: 10.1016/j.yfrne.2007.09.001.
  18. Pialek J., Vyskocilova M., Bamova B., Havelkova D., Pialkova J., Dufkova P., Bencova V., Dureje K., Albrecht T., Hauffe H.C., Macholan M., Munclinger P., Storchova R., Zajacova A., Hola V., Gregorova S., Forejt J. Development of unique house mouse resources suitable for evolutionary studies of speciation. J. Heredity. 2008; 99 (1): 34–44. DOI: 10.1093/jhered/esm083
  19. Stopkova R., Stopka P., Janotova K., Jedelska P.L. 2007. Species-specific expression of major urinary proteins in the house mice (Mus musculus musculus and Mus musculus domesticus). J. Chem. Ecol. 2007; 33 (4): 861–9. DOI: 10.1007/s10886-007-9262-9
  20. Tikhonova M.A., Amstislavskaya T.G. Phenotyping the males of mo.use and rat strains with genetically defined behavioral disturbances in a model of sexual activation. Russian Journal of Genetics: Applied Research. 2016; 6 (4): 410–6. DOI: 10.1134/S2079059716040201.
  21. Wysocki C.J., Katz Y., Bernhard R. Male vomeronasal organ mediates female-induced testosterone surges in mice. Biol. Reprod. 1993; 28 (4): 917–22.
  22. Wysocki C.J., Whitney G., Tucker D. Specific anosmia in the laboratory mouse. Behav. Genetics. 1977; 7 (2): 171–88.

You may be interested