Modeling Of Traumatic Injuries Of The Cornea Of The Eye

DOI: 10.29296/2618723X-2018-02-04

A. Ivkina(1), Researcher, St. Petersburg state chemical-pharmaceutical University, D. Yu.Ivkin(1), candidate of biological Sciences, head of St. Petersburg state chemical-pharmaceutical University, Е. Semivelichenko(1), Junior researcher St. Petersburg state chemical-pharmaceutical University, G. Plisko(1), Junior researcher St. Petersburg state chemical-pharmaceutical University, P. Burenkov(2), chief, preclinical research, «Samson-Med» 1-St. Petersburg state University of chemical and pharmaceutical Sciences Ministry of health of the Russian Federation, 197376, St. Petersburg, Professor Popov str., 14, lit. And 2-Samson-Med, 196158, St. Petersburg, Moscow highway, 13 E-mail: [email protected]

Keywords: traumatic erosion acid burn rabbits

For citation:

Ivkina A., Ivkin D., Semivelichenko E., Plisko G., Burenkov P. Modeling Of Traumatic Injuries Of The Cornea Of The Eye. Laboratory Animals for Science. 2018; 2. https://doi.org/10.29296/2618723X-2018-02-04

Abstract

On the center of experimental pharmacology Saint-Petersburg Chemical-Pharmaceutical University on rabbits male breed of Soviet chinchilla, as the most accessible and relevant test system, was conducted modeling of traumatic erosion by the method of S. Hanna, J.E. O’Brien (1960) and an acid burn of the cornea of the eye followed by a histological evaluation of the pathological process. The number of animals was sufficient to obtain statistically reliable results. The article describes in the great detail the conditions for keeping animals and reproducing models, a scoring of the severity of traumatic injury was suggested, which took into account the presence or absence of pathological changes, the presence and severity of the current exudative inflammation followed by pathological vascularization of the cornea, the presence and prevalence of fibroplastic changes in the cornea, pathological process and organization in its outcome. The consistency of the models is confirmed histologically, the severity of the lesion predominates in the modeling of traumatic erosion; in the future, the evaluation of the condition of the cornea may be of interest not only on the 29th day after the modeling of the pathology, but also on the 8th, 15th, 22nd days. In this case, it becomes possible to investigate the development of the pathological process in dynamics. These models are extremely relevant in the study of eye forms of drugs with regenerative, antihypoxic and antioxidant effects.

References

Kanyukov V.N., Stadnikov A.A., Trubina O.M., Yahina O.M. E`ksperimental`noe modelirovanie travmaticheskih povrezhdeniy rogovicy. – Vestnik OGU, 2014, 12 (173): 156–9.
Rukovodstvo po laboratornym zhivotnym i al`ternativnym modelyam v biomedicinskih tehnologiyah. Pod red.: N.N. Karkishhenko i S.V. Gracheva. M., 2010.
Rukovodstvo po provedeniyu doklinicheskih issledovaniy lekarstvennyh sredstv. Ch. I. M.: Grif i K, 2012.

You may be interested

Allergy to laboratory rodents: the underestimated problem
In the present review, we discuss the problem of hypersensitivity to laboratory animal allergens, which affects both animal caretakers and research personnel. Biological characteristics of the main allergens released by laboratory rodents are presented, as well a
Diet-induced models of metabolic disorders. Report 7: Experimental osteoporosis
The high incidence of osteoporosis dictates the need to find ways to effectively prevent and treat it, including in animal experiments.
Determination of reference intervals of creatinine clearance in laboratory animals
The assessment of renal function is an important part of monitoring the health of laboratory animals in preclinical studies. One of the most important indicators for the assessment of renal function is serum creatinine concentration and endogenous creatinine clearance.
Impedansometry as an estimating method of tertiary endpoint in experimental modeling of alimentary impairments
In preclinical practice, methods for assessing the body composition of laboratory animals are used, such as: computerized tomography, quantitative magnetic resonance, dual-energy X-ray absorptiometry, gravimetric method, bioimpedance spectroscopy method (hereinafter r
Comparative Morphology of Human and Laboratory Animals
Knowledge the characteristics of the morphological structure, the main structural and functional components of human skin and laboratory animals is the foundation of rational planning of pharmacological and toxicological preclinical studies of skin dosage forms in aspect.
Effect of deltametrin (Delcid) on the reproductive ability of laboratory mice
The key to successful research on laboratory animals is their quality. In the conditions of the domestic market of laboratory animals, there are a number of constraining factors, and it is almost impossible to purchase animals in the required volume.

Modeling Of Traumatic Injuries Of The Cornea Of The Eye

Abstract

References

Search

You may be interested