The study was supported by: MSU Institute of Mitoengineering, LLC; The program of basic research of the Institute of biomedical problems RAS (theme 65.4); Russian Laboratory Animal Science Association research grant (№ NI1, 01.03.2021) and MSU (scientific assignment № 121032300071–8). The funding sources took no part in the design, data collection, analysis, interpretation, or the decision to publish.
Andreev-Andrievsky A.A., Lagereva E.A., Pankova N.V., Mashkin M.A., Manskikh V.N., Frolova O.Yu., Fadeeva O.V., Telyatnikova E.V. Chronic bladder catheterization, but not the metabolic chambers, is suitable for collection of precise data on murine diuresis. Laboratory Animals for Science. 2022; 2. https://doi.org/10.29296/2618723X-2022-02-01
Metabolic cages (MC) are routinely used for urine collection from small laboratory rodents over long time intervals. In the case of urine collection from individual mice, the diuresis volume measured with MC is 20–40 ml/kg. If this value is used for water balance calculations, an underestimation of 60–80 ml/kg of the excreted water becomes apparent.
In a series of model studies with murine MCs, we show that volume recovery drops sharply in case liquid is introduced into the MC in small aliquots, approximating the murine voided volume (≈0.1 ml) due to evaporation. Evaporation occurs predominantly on the MC funnel, rather than from the urine collection tube surface. MC siliconization does not lead to substantial recovery improvement. The recovery is increased to acceptable values (≈80%) with higher aliquot volumes, thus group housing of mice in MCs is a feasible option to increase urine collection efficiency. The apparent limitation of group housing mice in MCs is the loss of data individuality. It should be mentioned that animals must be habituated to the MCs to minimize impact of stress on diuresis.
We have developed a new technique of urine collection in individual mice via the catheters chronically implanted into the bladder. The technique allows to precisely monitor diuresis in awake individual male and female mice for at least 30 days. Diuresis, as registered via the catheters, is 100.0±6.9 and 135.7±28.4 ml/kg in the males and females, correspondingly, which is almost thrice higher as compared to 39.0±5.4 and 37.2±3.2 ml/kg, collected from intact male and female mice with the MCs. The physicochemical properties of the catheter-collected urine are not distorted by evaporation, particularly, urine osmolarity was 2026±47 and 1870±41 mOsm in catheterized male and female mice, as compared to 2985±78 and 3413±105 mOsm in intact males and females in MCs.
The catheterization technique is suitable for a variety of physiological, pharmacological, and toxicological studies and has a minimal negative impact on the animals’ health.
Conflict of interest
The authors declare no conflicts of interest.
A.A. Andreev-Andrievsky — conception and design, data collection and analysis, drafting and reviewing the manuscript.
E.A. Lagereva — data collection and analysis, drafting and reviewing the manuscript.
N.V. Pankova — data collection and analysis, reviewing the manuscript.
M.A. Mashkin — data collection and analysis, reviewing the manuscript.
V.N. Mansky — data collection and analysis.
O.Yu. Frolova — data collection and analysis.
O.V. Fadeeva — data collection and analysis.
E.V. Telyatnikova — data collection and analysis.
All the authors have approved the version to be published.
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