Variability of blood biochemical parameters and establishment of reference intervals in preclinical studies. Part 5: ferrets

M.V. Miroshnikov, Candidate of Medical Sciences, Head of the Laboratory of Biochemistry and Hematology,
ORCHID 0000-0002-9828-3242;
K.T. Sultanova, Candidate of Medical Sciences, ORCHID 0000-0002-9846-8335;
M.A. Kovaleva, Candidate of Biological Sciences, Head of the scientific and methodological group,
ORCHID 0000-0002-0740-9357;
M.N. Makarova, MD, Director, ORCHID 0000-0003-3176-6386

Research and manufacturing company «Home оf Pharmacy»,
188663, Russia, Leningrad oblast, Vsevolozhskiy district, Kuzmolovskiy t.s., Zavodskaya st. 3-245

Е-mail: miroshnikov.mv@doclinika.ru

Abstract

Despite the relatively small number in preclinical studies, ferrets occupy a certain niche and have several unique features. Ferrets are used as models in the study of gastrointestinal and tumor diseases, cardiovascular pathology, as well as in research on the development of the nervous and visual systems. There is a significant similarity in the physiology and morphology of the lungs of ferrets and humans, and therefore this animal species is used as a model for studying cystic fibrosis, influenza and COVID-19. The purpose of this work is to summarize the results of biochemical blood parameters obtained in earlier studies on ferrets based on the NGO "House оf Pharmacy" in the period from January to June 2021 to establish reference values of the most frequently determined biochemical parameters.  A retrospective analysis of the data of 11 indicators was carried out.   A sample consisting of 112 females (56 males and 56 females) was analyzed, the body weight of females was 1200–1700 g, the body weight of males was 1500–1900 g. The animals were obtained from LLC "Novye Meha". During the adaptation period and subsequent experiments, the animals were kept in the same standard vivarium conditions: air temperature 22–26 ° C, relative humidity 40-75%, 12-hour daylight. Ferrets were fed in accordance with Directive 2010/63/EU of the European Parliament and of the Council of the European Union of September 22, 2010 on the protection of animals used for scientific purposes. The study was carried out in compliance with the principles of the European Convention for the Protection of Vertebrates Used for Experiments and Other Scientific Purposes (Strasbourg, 1986) and in accordance with the rules of good laboratory practice. Determination of biochemical parameters for all animals was carried out under equal conditions using generally accepted analytical methods. The following parameters were determined in the blood serum of animals: creatinine, urea, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, cholesterol, triglycerides, total protein, albumin, glucose and total bilirubin. When comparing the obtained and the literature data, it was shown that the reference values of biochemical parameters of ferrets calculated in JSC NPO Dom Pharmacy generally correlate with those of other scientific centers and laboratories, which confirms the reliability of the data obtained. The reference intervals of biochemical examination of ferret blood presented in the article confirm the relevance of periodic revision of indicators and indicate the importance of the results obtained for an adequate interpretation of the data obtained in the laboratory of biochemistry. A comparative analysis of the interindividual variability of biochemical parameters of ferret and human blood demonstrates the presence of species differences that must be taken into account when considering the results of preclinical studies.

Full text avaliable in Russain only 

Acknowledgements

The study was performed without external funding.

Authors’ contributions

M.V. Miroshnikov – analysis of scientific literature and guidelines, writing, editing and revision of the text, carrying responsibility for all aspects of the study related to the reliability of the data
K.T. Sultanova – writing and editing of the text, summarising the study results, preparation of the tables
M.A. Kovaleva – аnalysis of scientific literature and guidelines, editing and revision of the text.
M.N. Makarova – idea, planning of the study design, editing of the text

 

 

Conflict of interest

The authors declare no conflicts of interest.

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