Morphofunctional and histochemical features of the structure of the left lung of laboratory outbred albino rats

S.V. Zinoviev, Senior Researcher, Candidate of Medical Sciences, ORCID 0000-0001-6022-6130,
N.G. Plekhova, Head of the Central Research Laboratory, Doctor of Biological Sciences, ORCID 0000-0002-8701-7213,
I.V. Radkov, Junior Researcher, ORCID 0000-0001-6022-6130
Pacific State Medical University of the Ministry of Health of the Russian Federation

Е-mail: sinowev@mail.ru

Abstract

It is necessary to search for a method with which it is possible to extrapolate the indicators of the metabolic process to the organo-tissue level of lung organization in albino rats. Purpose of the study: to reveal the structural features of the left lung (LL) of outbred albino rats on the basis of histochemical analysis data. The experiment was carried out on sexually mature male Wistar rats (200–250 gr.). For the histochemical detection of calcium cations, the method of tissue staining with alizarin red C (ARC) and boric acid (BA) was used. After anesthesia and vivisection, the whole LL, extracted from the chest, was placed for 48 hours in a 5% ARC solution in 960 ethyl alcohol. Then the pieces of tissue were embedded in paraffin using chloroform solutions for dehydration. The sections obtained on a microtome were dewaxed in xylene and stained with an alcoholic solution of BA (5 min), after which they were examined under a fluorescent microscope.

Results. Supravital staining with alcoholic ARC solution in the stroma and on the surface of the alveoli of rat lungs reveals cells containing in the cytoplasm from 1 to 7 optically dense granules up to 3 μm in size. In the tissues of the lungs, basement membranes, cells containing structures with diol groups, are noted, and nuclei and nucleoli are visualized in them. In the middle sheath of the proximal generations and the caudal vein itself, fluorescent cardiomyocytes were observed, near which luminous thick connective tissue fibers were located. The distal veins of the respiratory parenchyma did not contain cardiomyocytes. The fluorescence of the bronchi stained with ARC and BA in the red range indicated an increased content of sugars and tyrosines, while the bright glow of the connective tissue fibers of the mucous membrane and basement membranes in the yellow-green range indicated a low content of tyrosine and sugars.

Thus, the method of processing LL tissues with staining of its tissues with ARC and BA allows for a clearer visualization of the structures of the bronchi, veins and arteries compared to the routine histological one.

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Author’s Contribution

Zinoviev S.V. – concept or design of the work; collection, analysis or interpretation of work results; writing text.

Plekhova N.G. – approval of the final version of the article for publication; agreement to be responsible for all aspects of the work, due study and resolution of issues related to the reliability of data or the integrity of all parts of the article.

Radkov I.V. – collection, analysis or interpretation of work results.

 

Conflict of interest

The authors declare no conflicts of interest.

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