Model of The Global Cerebral Ishemia in Mongolian Gerbil

DOI: 10.29296/2618723X-2019-02-03

V.Vavilova, Ya. Gushin

Research-and-manufacturing company «Home оf Pharmacy»
188663, Russia, Leningradskiy region, Vsevolozhskiy district, Kuzmolovskiy, Zavodskaya st., b. 245, 3
E-mail: [email protected]


Keywords: mongolian gerbil global cerebral ischemia stroke

For citation:

Vavilova V., Gushin Ya. Model of The Global Cerebral Ishemia in Mongolian Gerbil. Laboratory Animals for Science. 2019; 2. https://doi.org/10.29296/2618723X-2019-02-03

Abstract

The aim of this study was to develop a global cerebral ischemia simulation protocol (GCI, global cerebral ischemia) with subsequent reperfusion, which was carried out on Mongolian gerbil. The choice of a gerbil as a biological model is due to the anatomical feature of the blood supply to the brain. Gerbils, unlike other laboratory animals and humans, do not have a posterior communicating artery (do not have the full circle of Willis). Mortality, neurological status, pathological and histological studies were evaluated as criteria for the pathology degree estimation. Visualization of the ischemic region after the induction of pathology was carried out by staining brain sections with 2,3,5 triphenyl tetrazolium chloride (TTC). The method of the neurological status scoring which takes into account the presence or absence of pathological changes is described. The model is confirmed by pathological and histological examination. The severity of damage prevails on the 2nd and to a lesser extent on the 3rd day after the pathology induction. Histological examination of the gerbil brain identified neurons with signs of dystrophy (polymorphic, hyperchromic cells with fuzzy contours - “red” neurons) and reduced in size - “shadow cells”, as well as fields with mild gliosis in the cerebral cortex and cerebellar cortex. Thus, the results of the study showed that the 20-minute bilateral occlusion resulted in animal survival of about 60%, most animals demonstrated pronounced neurological deficit. Staining with TTC has shown that it is advisable to evaluate volume of the ischemic lesion in the first 24 hours after the induction of pathology. In addition, results showed that the neurological deficit was most pronounced during first 4 days after occlusion. To estimate long-lasting pathological changes it is advisable to conduct a histological examination of brain tissue samples.

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References

  1. Bacigaluppi M., Comi G., Hermann D. M. Animal models of ischemic stroke. Part two: modeling cerebral ischemia. The open neurology journal. 2010. Vol. 4: 34–8.
  2. Traystman R. J. Animal models of focal and global cerebral ischemia. Ilar j. 2003. Vol. 44; 2: 85–95.
  3. Ginsberg M. D., Busto R. Rodent models of cerebral ischemia. Stroke. 1989. Vol. 20; 12: 1627–42.
  4. Smith M. L., Bendek G., Dahlgren N., Rosen I., Wieloch T., Siesjo B. K. Models for studying long-term recovery following forebrain ischemia in the rat. 2. A 2-vessel occlusion model. Acta Neurol Scand. 1984. Vol. 69; 6: 385–401.
  5. Kato H., Araki T., Kogure K., Murakami M., Uemura K. Sequential cerebral blood flow changes in short-term cerebral ischemia in gerbils. Stroke. 1990. Vol. 21; 9: 1346–9.
  6. Tomida S., Nowak T. S., Jr., Vass K., Lohr J. M., Klatzo I. Experimental model for repetitive ischemic attacks in the gerbil: the cumulative effect of repeated ischemic insults. J Cereb Blood Flow Metab. 1987. Vol. 7; 6: 773–82.
  7. Levine S., Payan H. Effects of ischemia and other procedures on the brain and retina of the gerbil (Meriones unguiculatus). Exp Neurol. 1966. Vol. 16; 3: 255–62.
  8. Berry K., Wisniewski H. M., Svarzbein L., Baez S. On the relationship of brain vasculature to production of neurological deficit and morphological changes following acute unilateral common carotid artery ligation in gerbils. J Neurol Sci. 1975. Vol. 25; 1: 75–92.
  9. Brain imaging with correlative two-photon and light sheet microscopy. Costantini I., 2013.
  10. McGraw C. P. Experimental cerebral infarctioneffects of pentobarbital in Mongolian gerbils. Arch Neurol. 1977. Vol. 34; 6: 334–-6.
  11. Ohno K., Ito U., Inaba Y. Regional cerebral blood flow and stroke index after left carotid artery ligation in the conscious gerbil. Brain Res. 1984. Vol. 297; 1: 151–7.
  12. Rupadevi M., Parasuraman S., Raveendran R. Protocol for middle cerebral artery occlusion by an intraluminal suture method. J Pharmacol Pharmacother. 2011. Vol. 2; 1: 36–9.

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