A Comparative Study Of The Use Of High-Calorie Diets Enriched By Different Number Of Lipids For Modeling Metabolic Syndrome

M. Kovaleva, PhD, senior researcher, Ya. Guschin, Head of the Department of Histology and Pathology,, М. Makarova, PhD, MD, director, V. Makarov, professor, deputy director Research-and-manufacturing company «Houm of Pharmacy» Russia, 188663, Leningradskiy region, Vsevolozhskiy district, Kuzmolovskiy, st. Zavodskaya, 3. b. 245 Е-mail: kovaleva.ma@doclinika.ru

Abstract

Today, one of the main reasons for the spread of insulin resistance, which subsequently leads to the development of obesity, type 2 diabetes and metabolic syndrome (MS), is the peculiarity of modern human eating behavior, its propensity to unlimited intake of high-calorie food. Nutrition is an element of a lifestyle that can be controlled and thus directly affect the health of both the individual and the nation as a whole. In this regard, of particular interest is the simulation of MC in laboratory animals using high-calorie diets. The model of alimentary obesity allows to understand the reasons for the development and progression of MS and to investigate potential drugs for its prevention and treatment. The aim of this study was to compare the severity of manifestations of pathological changes in lipid and carbohydrate metabolism against the background of the experimental metabolic syndrome induced by diets enriched with different lipid content: D12451 (45% kcal from fat) and D12492 (60% kcal from fat) in laboratory mice. Since the 4th week of the study, a significant increase in body weight was observed in laboratory mice fed high-calorie diets enriched with lipids, which at the end of the study was about 10% in the D12451 group (45% kcal from fat) and 30% in the D12492 group (60% kcal from fat). A moderate hyperglycemia and a tendency to increase the activity of the enzyme dipeptidylpeptidase type 4 was revealed. At week 21, in the group of animals that received D12492 (60% kcal from fat) during the glucose tolerant test, development of glucose tolerance was established. On the background of application of both high-calorie fat diets in laboratory mice, structural and functional changes in the liver were revealed, which was reflected in a decrease in the glycogen content in the liver and development of large and small-dropped fatty liver disease. Our findings support show that diets enriched with lipids can be used by 45% and 60% to simulate the experimental metabolic syndrome in young 8weeks of age outbred male mice. The optimal timing of the formation of stable pathological changes characteristic of the alimentary metabolic syndrome was established. They were: 13 weeks - against the background of D12451 application - kcal from fat 45% and 17 weeks against the background of application D12492 - kcal from fat 60%. It should be noted that the use of a more high-calorie D12492 diet with a lipid content of 60% leads to metabolic disturbances not only of lipid but also carbohydrate metabolism, which is reflected in the development of glucose tolerance established during the glucose tolerant test.

References

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