high-speed video imaging method
native cytological material
rats of SPF-status
Barashkova S.V., Zhuravky S.G., Alekseenko S.I. Тhe Method Of Intravital Diagnosis Of Ciliary Activity Of Columnar Ciliated Epithelium Of The Nasal Mucosa In Laboratory Rats. Laboratory Animals for Science. 2018; 1. https://doi.org/10.29296/2618723X-2018-01-07
The results of a comparative study of the intravital cytological material of the columnar ciliated epithelium of the nasal cavity from intact adult rats of the Wistar (220-250 g) contained in the conventional type vivarium (n = 2) and SPF category (n = 2) are presented. An intact picture of cells of the columnar ciliated epithelium is noted in the material of SPF animals. A subacute inflammation and persistence of bacterial infection in animals of the conventional vivarium were observed. In this case, there were morphofunctional signs of degeneration of the ciliary apparatus: shortening, asynchronism and incomplete cycle of beating of the cilia. The best quality of the cytological material for in-vivo biopsy was obtained by using the combined cervical brushes «Cervex-Brush Combi» made of a polymer material modified by trimming the bristles of the central brush.
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