Variability of blood biochemical parameters and establishment of reference intervals in preclinical studies. Part 4: Mice

M.V. Miroshnikov, ORCID 0000-0002-9828-3242;
M.N. Makarova, ORCID 0000-0003-3176-6386

RMC «Home оf Pharmacy»
188663, Russia, Leningrad obl., Vsevolozhsky district, Kuzmolovsky t.s., Zavodskaya st. 3-245
Е-mail: miroshnikov.mv@doclinika.ru

Abstract

This article presents the results of the study and analysis of reference intervals of biochemical parameters of the blood of mice – one of the key test systems used at the preclinical stage of drug research. The data were obtained at the center of Scientific-Production Organisation «Номe of Pharmacy» in the period from January 2018 to December 2019 during the monitoring of animal health. The analyzed array contained data from 100 males and 100 females (non-pregnant and non-fertile females). During the adaptation period and subsequent experiments, the animals were kept in the same standard vivarium conditions: air temperature 22–26oC, relative humidity 40–75%, 12-hour daylight. The animals were fed in accordance with Directive 2010/63/EU of the European Parliament and of the Council of the European Union of September 22, 2010 on the protection of animals used for scientific purposes. The study was carried out in compliance with the principles of the European Convention for the Protection of Vertebrate Animals Used for Experiments and Other Scientific Purposes (Strasbourg, 1986) in accordance with the rules of good laboratory practice. The determination of biochemical parameters for all animals was carried out under equal conditions using analytical methods adopted in our testing center. The following parameters were determined in the blood serum of mice: creatinine, urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), cholesterol, triglycerides, total protein, albumin, glucose and total bilirubin. The data presented in the article indicate the need for periodic review of the reference intervals of laboratory animals used in experiments. The data obtained during the study confirm the need to create in-laboratory reference intervals in each specialized testing center not only for the mouse test system, but also for each animal species used in preclinical experiments, for a more accurate and detailed assessment of the clinical condition of the studied animals. The obtained reference intervals of the considered indicators correlate well with similar indicators from literary sources, which indirectly indicates their reliability.

Full text avaliable in Russain only 

Author’s Contribution

Miroshnikov M.V. – collection and systematization of material, statistical processing of the results, analysis and interpretation of the results, writing, editing text.
Makarova M.N. – idea, approval of the final version of the paper.

Conflict of interest

The authors declare that there is no conflict of interest.

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