Model of Double Infection by Trematode O. felineus (Rivolta, 1884) in C57BL/6J Mice

DOI: 10.29296/2618723X-2019-02-01

G. Vishnivetskaya, A. Katokhin, V. Naprimerov, E. Zavjyalov, D. Avgustinovich

Federal Research Center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences,
Lavretiev Ave., 10, Novosibirsk, 630090
E-mail: [email protected]; [email protected]

Keywords: C57BL/6J mice opisthorchiasis helminth O. felineus liver spleen blood
For citation:

Vishnivetskaya G., Katokhin A., Naprimerov V., Zavjyalov E., Avgustinovich D. Model of Double Infection by Trematode O. felineus (Rivolta, 1884) in C57BL/6J Mice. Laboratory Animals for Science. 2019; 2. https://doi.org/10.29296/2618723X-2019-02-01

Abstract

Opisthorchiasis is an infectious disease induced by the trematode larvae of the Opisthorchiidae family entering the gastrointestinal tract of mammals. Liver fluke Opisthorchis felineus (O. felineus) is the species of this family spreaded in Europe and the Russian Federation. Today, opisthorchiasis is considered as a systemic disease, in which not only the liver, as the main site of localization of parasites, is suffered, but also other organs and systems, including the central nervous system are affected. Therefore, the basic study of this parasitic infection with the modeling of opisthorchiasis on laboratory animals are needed. Chronic opisthorchiasis was induced in inbred C57BL/6J strain male mice with single or double administered metacercariae of O. felineus, obtained from infected ides caught from the Ob River in the Novosibirsk region. All experiments were performed in the SPF vivarium. Three groups of animals were compared: 1) uninfected control individuals (n=10), 2) single-infected by O. felineus larvae (n=12); 3) double-infected 3 months after the first invasion (n=18). The introduction of O. felineus larvae was carried out intragastrically with the help of specialized probes. After 6 months from the start of the experiment, a greater number of helminths in the liver of the double-infected mice were found compared to the single-infected ones. Moreover, an increase in the number of helminths was due to presence of the immature parasites, whereas the size of the mature ones varied from 1.5 to 3 mm. Under the background of double infection, an increased negative impact of helminths on the host physiological state was established. These animals showed raised alanine aminotransferase enzyme activity (ALT) in serum, which is the main marker of inflammatory processes in the liver. In addition, the decreased body weight and hepatosplenomegaly were observed in mice by the end of the experiment. As for a single infection, the increase in weight indices of the liver and spleen were less pronounced, and the activity of ALT did not differ from control values. It was concluded that mice of the C57BL/6J inbred strain can be used for modeling of opisthorchiasis in the laboratory conditions, moreover the double infection having a more pronounced negative effect on the host state.

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