Ustenko Zh., Petlitskaya S., Gushchina S., Gushchin Ya., Makarova M. THE EFFECT OF FIXING SOLUTIONS ON THE SEVERITY OF ARTIFACTS IN THE RETINA AND CHOROID IN HISTOLOGICAL SECTIONS OF THE RABBIT EYE. Laboratory Animals for Science. 2022; 1. https://doi.org/10.29296/2618723X-2022-01-04
Rabbits are often chosen as animal models for the study of eye pathologies, including pathologies of the retina and choroid. Fixation of the eyeball of a rabbit is a difficult task. In the process of fixation, many artifacts occur in it. Many factors influence the frequency of occurrence and severity of artifacts: the procedure for selecting the eyeball, the type of fixative fluid, and the cutting method. The most common artifacts in the study of histological sections of the eyes of rabbits include separation of neuroretina from the pigment epithelium, separation in the choroid, separation between the choroid and sclera, bleb-like protrusions of the nerve fiber layer into the vitreous. The current study presents the result of the evaluation the effect of the type of fixation fluid on the severity of the main artifacts in the retina and choroid of the eyes of rabbits. We studied the effect of 4 types of fixing fluid: 10% neutral formalin, Davidson's solution, freshly prepared 4% paraformaldehyde solution, freshly prepared 2% paraformaldehyde solution.
Retinal separation was noted in all samples on macroscopic examination. Macroscopically the greatest severity of the separation was detected in samples fixed in 10% formalin solution. Histological examination showed that samples fixed in freshly prepared 2% paraformaldehyde solution had greatest severity of the separation of neuroretin from the pigment epithelium (p=0.0009). Samples fixed in 10% neutral formalin solution had greatest severity of the separation of the choroid (p=0.0004). There was no statistically significant effect of the type of fixative fluid on the severity of separation of the choroid from the sclera (p=0.0750). Bleb-like protrusions of the nerve fiber layer was found in groups fixed in freshly prepared 4% paraformaldehyde solution, 10% formalin solution and Davidson's solution once. The statistically significant was found between the osmolality of the fixing solution and the severity of retinal dissection in histological sections. Spearman correlation coefficient – 0.556 (p<0.05). No statistically significant relationship was found between the osmolality of the fixing solution and the severity of other artifacts. Spearman correlation coefficient – -0.218 and -0.148 respectively (p>0.05).
The study was performed without external funding.
Zh. Ustenko – design of the research, conducting research, systematization of material, writing the article, providing of the photo mateials.
S. Petlitskaya – conducting research, systematization of material.
S. Gushchina – data analysis and statistical processing.
Ya. Gushchin – idea, concept of the research, editing the article.
M. Makarova – editing and approval of the final version of the article for publication.
The authors declare no conflicts of interest.
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