The methods of visualization and research of the git-associated lymphoid tissue of laboratory animals

DOI: 10.29296/2618723X-2020-03-09

E.V. Belyaeva, veterinarian pathologist of the department of histology and pathomorphology,
Ya.A. Guschin, Head of the department of Histology and Pathology

Research-and-manufacturing company «HOME OF PHARMACY»

188663, Russia, Leningradskiy region, Vsevolozhskiy district, Kuzmolovskiy, Zavodskaya st., 3-245

Е-mail: [email protected]

Keywords: immune system gut-associated lymphoid tissue necropsy laboratory animals pathomorphology
For citation:

Belyaeva E.V., Guschin Ya.A. The methods of visualization and research of the git-associated lymphoid tissue of laboratory animals. Laboratory Animals for Science. 2020; 3. https://doi.org/10.29296/2618723X-2020-03-09

Abstract

The immune system is an important part of the vital activity in the organism, because it prevents a penetration of foreign bodies and substances or it deletes them very quickly. Its elements can be found throughout in all organ systems in the form of various lymphoid formations or individual cells. The mucosa-associated lymphoid tissue (MALT) is one of the important parts of the immune system. The gut-associated lymphoid tissue (GALT) is the largest department of MALT, because about 80% of all defensive cells contact with the mucous membrane of the intestine. Some toxic effects on the organism show expression or suppression of immune responses of a body or don’t appear. So thorough lymphoid tissue analysis is a very important part in the preclinical study. The purpose of this study is to detect of existing visualization methods and research of the GALT and a selection of the most optimal methods for their usage in experiments. Some macroscopic research methods were considered, one of which was put into practice. The organs of male and female rats and rabbits were used in the study. A detailed description of visualization methods has not been preserved in literary sources, so the most optimal method to stain native intestinal preparations for macroscopic examination of intestinal associated lymphoid tissue was developed in experiments. The most informative method of the histological examination also was described and shown in this research, which one allows to study and the GALT and the intestinal mucosa. In this case, you need to select all the sections of the intestine, so that can be explored on all surface of the intestinal mucosa, and you can also examine in detail all the lymphoid formations of the intestine under a microscope. The results will help to expand the methods of visualisation and researching of the immunotoxicity of various drugs in preclinical studies. In addition, the results can be used in a detailed study of the structure of the immune system of an animal’s organism.

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References

  1. Морозова Е.Н., Морозов В.Н., Кузьмачук Д.О., Моргун Ю.А. Взгляд на морфогенез пейеровых бляшек тонкой кишки крыс// Вестник проблем биологии и медицины. Украина. 2013. №2, том 2 (101). С. 27-32 [Morozova E.N., Morozov V.N., Kuz'machuk D.O., Morgun Yu.A. Vzglyad na morfogenez peierovykh blyashek tonkoi kishki krys// Vestnik problem biologii i meditsiny. Ukraina. 2013. №2, tom 2 (101). S. 27-32 (In Russ)].
  2. Воронцова З.А., Никитюк Д.Б., Кудаева Э.Ф. Кишечно-ассоциированная лимфоидная ткань как информационно-корректирующая система экстремальных состояний// Вестник новых медицинских технологий, 2016. №4. 289-294. DOI: 10.12737-21854 [Vorontsova Z.A., Nikityuk D.B., Kudaeva Eh.F. Kishechno-assotsiirovannaya limfoidnaya tkan' kak informatsionno-korrektiruyushchaya sistema ehkstremal'nykh sostoyanii// Vestnik novykh meditsinskikh tekhnologii, 2016. №4. 289-294. DOI: 10.12737-21854 (In Russ)].
  3. Sminia T., Jeurissen S.H.M. Gut-Associated Lymphoid Tissue, Rodent, Normal Structure and Function. In: Jones T.C., Ward J.M., Mohr U., Hunt R.D. (eds). Hemopoietic System. Monographs on Pathology of Laboratory Animals. Springer. Berlin. Heidelberg. 1990 DOI: 10.1007/978-3-642-84110-1_48
  4. Stamvorth D. R Current concepts in hypersensitivity - – In: Immunotoxicology and Immunopharmacology /Eds. Dean J. H. et al. New York: Raven Press. 1985. P. 91-99.
  5. A. J. Awang-Hazmi, A. B. Z. Zuki, M. Zamri-Saad [et al.] The response of Gut Associated Lymphoid Tissues (GALT) following oral administration of P. Multocida B2 in rats // Priory Medical Journal. - – 2007. - – № 56. - – P. 1039-1045.
  6. Miller M.J., Newberry R.D. Microanatomy of the intestinal lymphatic system // NIH Public Access, 2011. – 1207(Suppl 1). – P. 21-28.
  7. Гусейнова С.Т. Морфологические изменения в лимфоидных узелках тонкой кишки при дегидратации // Морфология, 2010. №75. – С. 44-46 . [Guseinova S.T. Morfologicheskie izmeneniya v limfoidnykh uzelkakh tonkoi kishki pri degidratatsii // Morfologiya, 2010. №75. – S. 44-46 (In Russ)]
  8. Balmasova I. P., Sepiashvili R. I. Intestine infections, inflammation and autoimmunity. Lymphoid apparatus of intestine in interaction with intestine microflora // Zh Mikrobiol Epidemiol Immunobiol. – 2013. – № 1. – Р. 113-120.
  9. Норматов, Р. А., Марьяновская Ю. В. Лимфоидная ткань кишечника как основа иммунной системы пищеварительного тракта // Молодой ученый. — 2017. — № 20 (154). — С. 201-203 [Normatov, R. A., Mar'yanovskaya Yu. V. Limfoidnaya tkan' kishechnika kak osnova immunnoi sistemy pishchevaritel'nogo trakta // Molodoi uchenyi. — 2017. — № 20 (154). — S. 201-203 (In Russ)].
  10. Ленченко Е.М. Цитология, гистология и эмбриология // М.: «Юрайт», 2018. – 347 с [Lenchenko E.M. Tsitologiya, gistologiya i ehmbriologiya // M.: «YuraiT», 2018. – 347 s (In Russ)].
  11. Рыбакова А.В., Макарова М.Н. Методы эвтаназии лабораторных животных в соответствии с европейской директивой 2010/63 // Международный вестник ветеринарии. 2015. N2.С. 96-107 [Rybakova A.V., Makarova M.N. Metody ehvtanazii laboratornykh zhivotnykh v sootvetstvii s evropeiskoi direktivoi 2010/63 // Mezhdunarodnyi vestnik veterinarii. 2015. N2.S. 96-107 (In Russ)].
  12. Директива 2010/63/EU Европейского парламента и совета Европейского союза от 22.09.2010 по охране животных, используемых в научных целях. Гл. I, ст. 6 [Direktiva 2010/63/EU Evropeiskogo parlamenta i soveta Evropeiskogo soyuza ot 22.09.2010 po okhrane zhivotnykh, ispol'zuemykh v nauchnykh tselyakh. Gl. I, st. 6 (In Russ)].
  13. Hellman Т. Stadies uber der Lymphoide die Bedeutung der Sekundar-follikle // Beitr. z. Pathol. Anat. U. Aug. Path. 1921. – Bd. 68. 5. – P.335-365.
  14. Кащенко С.А. Выявление морфологических особенностей лимфоидных образований тонкой кишки при окраске нативного препарата / Мир медицины и биологии // Украина, 2011. – №1. – С. 27 – 29 [Kashchenko S.A. Vyyavlenie morfologicheskikh osobennostei limfoidnykh obrazovanii tonkoi kishki pri okraske nativnogo preparata / Mir meditsiny i biologii // Ukraina, 2011. – №1. – S. 27 – 29 (In Russ)].
  15. Оленева Е.В., Четверных В.А., Костицын А.С., Пономарева С.В., Мякишева Ю.В. Патент СССР №SU 1 680 088 A1, 1991.09.30. Способ выявления микроциркуляторного русла и лимфоидной ткани кишечника // 4461629, 1988.07.15 [Oleneva E.V., Chetvernykh V.A., Kostitsyn A.S., Ponomareva S.V., Myakisheva Yu.V. Patent SSSR №SU 1 680 088 A1, 1991.09.30. Sposob vyyavleniya mikrotsirkulyatornogo rusla i limfoidnoi tkani kishechnika // 4461629, 1988.07.15 (In Russ)]
  16. Жданова О.Б., Ашихмин С.П., Параманова А.Н. [и др.] Новые методологические решения в патоморфологии гельминтозов // Российский паразитологический журнал, 2010. – № 4. – С. 74–82 [Zhdanova O.B., Ashikhmin S.P., Paramanova A.N. [i dr.] Novye metodologicheskie resheniya v patomorfologii gel'mintozov // Rossiiskii parazitologicheskii zhurnal, 2010. – № 4. – S. 74–82 (In Russ)].
  17. Кокорина А.Е., Беспятых О.Ю. Макроанатомические изменения лимфоидной ткани тонкого кишечника лисицы и песца под влиянием янтарной кислоты // Аграрная наука Евро-Северо-Востока, 2011. – №5 (24). – С. 49-53 [Kokorina A.E., Bespyatykh O.Yu. Makroanatomicheskie izmeneniya limfoidnoi tkani tonkogo kishechnika lisitsy i pestsa pod vliyaniem yantarnoi kisloty // Agrarnaya nauka Evro-Severo-Vostoka, 2011. – №5 (24). – S. 49-53 (In Russ)].
  18. Moolenbeek C., Ruitenberg E.J. The «Swiss roll»: a simple technique for histological studies of the rodent intestine // Laboratory Animals, 1981. – №15. – P. 57-59.
  19. Bialkowska A. B., Nandan M., Ghaleb Amr. M., Yang V.W. Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses // Journal of Visualized Experiments, 2016. – №113.
  20. Мужикян А.А., Макарова М.Н., Гущин Я.А. Особенности гистологической обработки органов и тканей лабораторных животных. // Международный вестник ветеринарии, 2014. – № 2. – С. 103-109 [Muzhikyan A.A., Makarova M.N., Gushchin Ya.A. Osobennosti gistologicheskoi obrabotki organov i tkanei laboratornykh zhivotnykh. // Mezhdunarodnyi vestnik veterinarii, 2014. – № 2. – S. 103-109 (In Russ)].
  21. Манских В.Н. Патоморфология лабораторной мыши: в 3-х т.; Т. 1. Технические аспекты. Общая и органная патология. – М.:ВАКО, 2016. – 208 с [Manskikh V.N. Patomorfologiya laboratornoi myshi: v 3-kh t.; T. 1. Tekhnicheskie aspekty. Obshchaya i organnaya patologiya. – M.:VAKO, 2016. – 208 s (In Russ)].

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