The immune system is an important part of the vital activity in the organism, because it prevents a penetration of foreign bodies and substances or it deletes them very quickly. Its elements can be found throughout in all organ systems in the form of various lymphoid formations or individual cells. The mucosa-associated lymphoid tissue (MALT) is one of the important parts of the immune system. The gut-associated lymphoid tissue (GALT) is the largest department of MALT, because about 80% of all defensive cells contact with the mucous membrane of the intestine. Some toxic effects on the organism show expression or suppression of immune responses of a body or don’t appear. So thorough lymphoid tissue analysis is a very important part in the preclinical study. The purpose of this study is to detect of existing visualization methods and research of the GALT and a selection of the most optimal methods for their usage in experiments. Some macroscopic research methods were considered, one of which was put into practice. The organs of male and female rats and rabbits were used in the study. A detailed description of visualization methods has not been preserved in literary sources, so the most optimal method to stain native intestinal preparations for macroscopic examination of intestinal associated lymphoid tissue was developed in experiments. The most informative method of the histological examination also was described and shown in this research, which one allows to study and the GALT and the intestinal mucosa. In this case, you need to select all the sections of the intestine, so that can be explored on all surface of the intestinal mucosa, and you can also examine in detail all the lymphoid formations of the intestine under a microscope. The results will help to expand the methods of visualisation and researching of the immunotoxicity of various drugs in preclinical studies. In addition, the results can be used in a detailed study of the structure of the immune system of an animal’s organism.
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