Variability of blood biochemical parameters and establishment of reference intervals in preclinical studies. Part 3: mini pigs

N.G. Voitenko, ORCID 0000-0002-3164-4971,   

M.N. Makarova, ORCID 0000-0003-3176-6386

Research and manufacturing company «Home оf Pharmacy»,

188663, Russia, Leningrad oblast, Vsevolozhskiy district, Kuzmolovskiy t.s., Zavodskaya st. 3-245

Е-mail: [email protected]

Abstract

. For today, scientists have more than a dozen other laboratory animals at their disposal to conduct toxicological, pharmacokinetic and other studies in addition to rodents. Pigs have been used for a long time to study organ and tissue transplants and surgical training for medical students, but their use can be much broader. The anatomical similarity to humans and the large size of these animals allow us to successfully conduct studies of the cardiovascular, digestive and urinary systems, as well as use them in preclinical and Toxicological studies. In this regard, the establishment of reference intervals of biochemical blood parameters of laboratory pigs is an urgent task.

Our article presents reference intrevals of biochemical parameters of blood of dwarf pigs obtained using the classical approach. We used the results of biochemical blood analysis of 15 males and 15 females of clinically healthy dwarf pigs aged 9-11 months, weighing 14-26 kg, raised in our nursery. In addition to the main biochemical parameters (creatinine, urea, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, cholesterol, triglycerides, total protein, albumin, glucose, and total bilirubin), additional parameters are presented – an expanded lipid profile, calcium and phosphorus concentrations, and the activity of a number of enzymes (lactate dehydrogenase, creatine kinase, amylase, gamma-glutamyltransferase, and cholinesterases), which are biochemical markers of damage to various systems and organs.

The most pronounced differences were found in the study of the lipid profile of male and female dwarf pigs: the concentration of total cholesterol and triglycerides in the blood of females was higher on average by 1.5 and 2 times, respectively. In addition, the concentration of low-density lipoprotein cholesterol in the blood serum of females was on average 1.8 times higher than in the blood serum of males. Less pronounced but statistically significant differences were found in the concentration of total protein and globulins.

The analysis of the presented reference intervals with the literature data showed the comparability of the obtained values. Despite the significant similarity of the biochemical profiles of humans and laboratory pigs, it is necessary to take into account the presence of species differences when considering the results of preclinical studies.

Full text avaliable in Russain only

Authors’ сontributions

Natalya.G. Voitenko – idea, planning, design development, collection and systematisation of material, analysis and interpretation of results, writing and revising the text.

Marina N. Makarova – idea, design development.

References

  1. Kim H.I., Lee S.Y., Jin S.M., Kim K.S., Yu J.E., Yeom S.C., Yoon T.W., Kim J.H., Ha J., Park C.G., Kim S.J. Parameters for successful pig islet isolation as determined using 68 specific-pathogen-free miniature pigs Xenotransplantation. 2009 16(1):11-8.
  2. Wang S., Liu Y., Fang D., Shi S. The miniature pig: a useful large animal model for dental and orofacial research. Oral Dis. 2007 13(6):530-7.
  3. Walters E.M., Prather R.S. Advancing Swine Models for Human Health and Diseases Mo Med. 2013; 110(3): 212–15.
  4. Smith A.C., Swindle M.M. Preparation of swine for the laboratory. ILAR J. 2006;47(4):358–363.
  5. Lorson M.A., Spate L.D., Samuel M.S., Murphy C.N., Lorson C.L., Prather R.S., et al. Disruption of the Survival Motor Neuron (SMN) gene in pigs using ssDNA. Transgenic Res. 2011;20:1293-1304.
  6. Swanson K.S., Mazur M.J., Vashisht K., Rund L.A., Beever J.E., Counter C.M., et al. Genomics and Clinical Medicine: Rationale for Creating and Effectively Evaluating Animal Models. Experimental Biology and Medicine. 2004;229:866–75
  7. Welsh M.J., Rogers C.S., Stoltz D.A., Meyerholz D.K., Prather R.S. Development of a porcine model of cystic fibrosis. Trans Am Clin Climatol Assoc. 2009;120:149–162.
  8. Rogan M.P., Reznikov L.R., Pezzulo A.A., Gansemer N.D., Samuel M., Prather R.S., et al. Pigs and humans with cystic fibrosis have reduced insulin-like growth factor 1 (IGF1) levels at birth. Proc Natl Acad Sci U S A. 2010;107:20571–20575.
  9. Animal Research Numbers in 2019. – URL.: https://www.understandinganimalresearch.org.uk/news/communications-media/animal-research-numbers-in-2019, (дата обращения 07.08.2020 г).
  10. TUKEY, J.W. Exploratory data analysis. Reading, Massachusetts: Addison-Wesley, 1977
  11. Маршалл В.Дж. Клиническая биохимия/ Пер. с англ. – М. – Спб.: «Издательство БИНОМ» - «Невский Диалект», 1999. – 368 с. [Marshall V.Dzh. Klinicheskaya biokhimiya/ Per. s angl. – M. – Spb.: «Izdatel'stvo BINOM» - «Nevskij DialekT», 1999. – 368 s.]
  12. Yeom S.C., Cho S.Y., Park C.G., Lee W.J. Analysis of reference interval and age-related changes in serum biochemistry and hematology in the specific pathogen free miniature pig Lab Anim Res. 2012 28(4):245-53
  13. Fox J.G. Laboratory Animal Medicine. Academic Press; Amsterdam, The Netherlands; New York: 2002.
  14. Helke K.L., Ezell P.C., Duran-Struuck R., Swindle M.M. Biology and Diseases of Swine. Laboratory Animal Medicine, Third Edition Elsevier Inc 2015
  15. Swindle M.M. Swine in the Laboratory: Surgery, Anesthesia, Imaging, and Experimental Techniques. CRC Press; Boca Raton, FL: 2007
  16. Badin J.K., Kole A., Stivers B., Progar V., Pareddy A., Alloosh M., and Sturek M. Alloxan-induced diabetes exacerbates coronary atherosclerosis and calcification in Ossabaw miniature swine with metabolic syndrome J Transl Med. 2018; 16: 58.
  17. Minipigs, E.G., 2010. The Gottingen Minipig [Online]. – URL: http://minipigs.dk/thegottingenminipig/ (дата обращения 10.08.2020).

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