New in the science and practice of preclinical research. Report 2

M. Makarova, ORCID 0000-0003-3176-6386.
V. Makarov, ORCID 0000-0002-2447-7888

St. Petersburg Institute of Pharmacy

188663, Russia, Leningradskiy region, Vsevolozhskiy district, Kuzmolovskiy t.s., Zavodskaya st. 3–245

Е-mail:  [email protected]

Abstract

The article analyzes a number of important publications for 2019 for scientists engaged in biomedical research. Thus, a new model of melanoma in mice of the c-Kit-CreER line with tamoxifen is presented, which will provide an opportunity to test new chemotherapeutic and immunotherapy methods of treatment, as well as the potential health risks associated with them. In another publication, scientists from San Francisco who are studying the mechanisms of aging based on the study of the immunity of the naked mole rat, whose life span is ten times longer than that of ordinary rats, came to the conclusion about the significance of this high number of neutrophils, which play an important role in innate and adaptive immunity.

An article by American scientists published in the journal of the American Association for Laboratory Animal Science in November 2019 is devoted to the analysis of the most commonly used methods of handling mice when manipulating them (cleaning cells, moving animals, etc.), such as the use of forceps (tweezers), gloved hands, plastic cups and special tunnels. In the July issue of the same journal, materials are provided to justify the possibility of increasing the interval of sanitary treatment of the upper parts (covers) of mouse cells to 90 days without compromising the sanitary condition and health of animals, in contrast to cells for rats, which must be treated at least once every 2 weeks. This reduces the labor cost of caring for mice and increases the service life of the equipment.

The possibility of reducing the time of sanitary treatment of mouse cells in animal care programs is discussed in the September issue of the same journal. At the same time, the authors based on the Lean Six Sigma methodology (process optimization methodology) developed specific recommendations, such as: purchasing additional cells and racks for them; drawing up clear daily work schedules for each employee, taking into account specific stages of work; marking on the floor with colored tape areas of clean and dirty processes, as well as ways to move cells and racks in order to ensure that they do not intersect. This reduced the average cleaning time per rack of mouse cells by 35 minutes, and the time saved was used to improve the health and living conditions of laboratory animals.

Full text avaliable in Russain only

Acknowledgements

The study was performed without external funding.

Authors’ contributions 

Marina N. Makarova – idea. analysis of literature data, compilation of the list of references. writing, revising the text.

Valeriy G. Makarov – data collection and systematization, writing individual parts of the text.

Conflict of interest

The authors declare no conflict of interest.

References

  1. Sun Q., Lee W., Mohri Y., Takeo M., Lim C.H., Xu X., Myung P., Atit R.P., Taketo M.M., Moubarak R.S., Schober M., Osman I., Gay D.L., Saur D., Nishimura E.K., Ito M. A novel mouse model demonstrates that oncogenic melanocyte stem cells engender melanoma resembling human disease. Nat. Commun., 2019; Nov 4; 10 (1):5023. – 16 p. doi: 10.1038/s41467-019-12733-1.
  2. Hilton H.G., Rubinstein N.D., Janki P., Ireland A.T., Bernstein N., Fong N.L., Wright K.M., Smith M., Finkle D., Martin-McNulty B., Roy M., Imai D.M., Jojic V., Buffenstein R. Single-cell transcriptomics of the naked mole-rat reveals unexpected features of mammalian immunity. PLoS Biol. 2019 Nov; 17(11): e3000528. doi: 10.1371/journal.pbio.3000528
  3. Doerning C.M., Thurston S.E., Villano J.S., Kaska C.L., Vozheiko T.D., Soleimanpour S.A., Lofgren J.L. Assessment of Mouse Handling Techniques During Cage Changing. J. Am. Assoc. Lab. Anim. Sci., 2019 Nov 1; 58 (6): 767-773. doi: 10.30802/AALAS-JAALAS-19-000015.
  4. Esvelt M.A., Steiner L., Childs-Thor C., Dysko R.C., Villano J.S., Freeman Z.T. Variation in Bacterial Contamination of Microisolation Cage Tops According to Rodent Species and Housing System. J. Am. Assoc. Lab. Anim. Sci., 2019 Jul 1;58(4):450-455. doi: 10.30802/AALAS-JAALAS-18-000126.
  5. Guide for the care and use of laboratory animals, 8th ed. Institute for Laboratory Animal Research. 2011. Washington (DC): National Academies Press.
  6. Kovach J.V., Dash A. Using the Lean Six Sigma Methodology to Reduce Mouse Cage Sanitation Time for Animal Care and Use Programs. J. Am. Assoc. Lab. Anim. Sci., 2019 Sep 1; 58 (5): 551-557. doi: 10.30802/AALAS-JAALAS-18-000121

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