Variability of blood biochemical parameters and establishment of reference intervals in preclinical studies. Part 2: rabbit

DOI: 10.29296/2618723X-2020-02-01

N.G. Voitenko, M.N. Makarova, M.A. Kovaleva

Е-mail: [email protected]

Keywords: reference intervals of biochemical indicators normal values of biochemical indicators new zealand rabbit chinchilla white giant
For citation:

Voitenko N.G., Makarova M.N., Kovaleva M.A. Variability of blood biochemical parameters and establishment of reference intervals in preclinical studies. Part 2: rabbit. Laboratory Animals for Science. 2020; 2. https://doi.org/10.29296/2618723X-2020-02-01

Abstract

The data of the biochemical analysis are the reference for most of the preclinical studies. The establishment and revision of reference ranges of biochemical indicators for all types of used laboratory animals is an important task for each testing center. In the Russian Federation, rabbits are widely used for various studies, for example, the study of the general toxic properties of original drugs, or long-acting drugs, the study of the specific activity of drugs used for the treatment of the visual organs and the musculoskeletal system, etc. Despite the sufficient study of the species as a whole, Russian periodicals present a limited amount of data on the reference intervals of biochemical indicators. Our test center has accumulated a data bank of such indicators that were determined in multiple-dose toxicity studies, which allowed us to conduct a retrospective analysis and review the available reference intervals. The calculation was based on observation data obtained in the period from January 2018 to December 2019. The determination of biochemical parameters in this period of time was carried out on the same equipment using the same methods. A retrospective analysis of the data was performed on 13 biochemical parameters of blood serum of three breeds of rabbits – New Zealand, chinchilla, white giant. The sample consisted of 127 females and 127 males, the age of the animals in the studies was 16–25 weeks (the weight of the animals was 3–5 kg). The article presents reference intervals for creatinine, urea, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, cholesterol, triglycerides, total protein, albumin, globulin, glucose, total bilirubin, and the albumin/globulin ratio. The necessity of creating intra-laboratory databases of reference intervals in each test center is justified. Interindividual variability was calculated for the specified biochemical parameters. The greatest inter-individual variability (more than 30%) was found for such indicators as alkaline phosphatase, triglycerides, cholesterol and total bilirubin. The use of retrospective data analysis allows us to implement the principles of 3Rs and get more correct data on a larger sample of laboratory animals. The analysis of the reference intervals obtained by us in comparison with the literature data, in general, showed their comparability, which can be considered as an additional confirmation of the satisfaction of the established intra-laboratory standards.

Authors’ сontributions

Natalya.G. Voitenko – idea, planning, design development, collection and systematisation of material, writing and revising the text.

Marina N. Makarova – idea, design development.

Mariya A. Kovaleva – analysis and interpretation of results, writing and revising the text

 

Full text avaliable in Russain only

Conflict of interest

The authors declare no conflict of interest requiring disclosure in this article.

References

  1. Animal Research Numbers in 2018. Дата обращения 13.04.2020, URL.: http://www.understandinganimalresearch.org.uk/news/communications-media/animal-research-numbers-in-2018/
  2. Щелкунова Е.И., Воропаева А.А., Русова Т.В., Штопис И.С.В. Применение экспериментального моделирования при изучении патогенеза остеоартроза (обзор литературы) // Сибирский научный медицинский журнал. – 2019; 39 (2): 27–39. [Shchelkunova E.I., Voropaeva A.A., Rusova T.V., Shtopis I.S.V. Primenenie eksperimental'nogo modelirovaniya pri izuchenii patogeneza osteoartroza (obzor literatury) //sibirskii nauchnyi meditsinskii zhurnal. – 2019; 39, № 2: 27–39. (In Russ.)].
  3. Yoshioka M., Coutts R.D., Amiel D., Hacker S.A. Characterization of a model of osteoarthritis in the rabbit knee // Osteoarthritis Cartilage. – 1996; 4 (2): 87–98.
  4. Dornas W.C., Oliveira T.T., Augusto L.E., Nagem T.J. Experimental atherosclerosis in rabbits // Arq Bras Cardiol. – 2010; 95 (2): 272–278. DOI: 10.1590/s0066-782x2010001200020
  5. Xiao X., Pei L., Jiang L.J., Lan W.X., Xiao J.Y., Jiang Y.J., Wang Z.Q. (2018) In Vivo Pharmacokinetic/ Pharmacodynamic Profiles of Danofloxacin in Rabbits Infected With Salmonella typhimurium After Oral Administration // Front. Pharmacol. – 2018; 9: 1–8 DOI: 10.3389/fphar.2018.0039.
  6. Теория статистики: учебное пособие / Л.Г. Батракова. – М.: КНОРУС, 2016. – 528 с. [Teoriya statistiki: uchebnoe posobie / L.G. Batrakova. – M.: KNORUS. – 2016; 528 p. (In Russ.)].
  7. ГОСТ Р53022-3-2008. Технологии лабораторные и клинические. Требования к качеству лабораторных исследований. Правила оценки клинической эффективности лабораторных тестов. – М., 2008. [GOST R53022-3-2008. Tekhnologii laboratornye i klinicheskie. Trebovaniya k kachestvu laboratornykh issledovanii. Pravila otsenki klinicheskoi effektivnosti laboratornykh testov. – M., 2008. (In Russ.)]
  8. ГОСТ Р 53022.2-2008 Технологии лабораторные клинические. Требования к качеству клинических лабораторных исследований. Ч. 2. Оценка аналитической надежности методов исследования (точность, чувствительность, специфичность). [GOST R 53022.2-2008 Tekhnologii laboratornye klinicheskie. Trebovaniya k kachestvu klinicheskikh laboratornykh issledovanii. Chast' 2. Otsenka analiticheskoi nadezhnosti metodov issledovaniya (tochnost', chuvstvitel'nost', spetsifichnost'). (In Russ.)]
  9. Clinical and Laboratory Standards Institute. Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory; Approved Guideline. 3rd Ed. – Wayne, PA, 2008.
  10. Suckow M.A., Schroeder V., Douglas F.A. The Laboratory Rabbit 2nd Edición, Edición Kindle. – 2012; 150 р.
  11. Özkan C., Kaya A., Akgül Y. Normal values of haematological and some biochemical parameters in serum and urine of New Zealand White rabbits // World Rabbit Sci. – 2012; 20: 253–259 DOI:10.4995/wrs.2012.122.
  12. Black D.M., Gilardi K.V.K., Hamilton L.P., Williams E., Williams D. F., Kelly P.A., Gardner I. Hematologic and biochemistry reference values for the endangered riparian brush rabbit (Sylvilagus Bachmani Riparius) // Journal of Wildlife Diseases. – 2009; 45 (2): 491–496.
  13. Миронова И.В., Черненков Е.Н., Черненков А.А. Показатели крови кроликов при включении в рацион пробиотической кормовой добавки Биогумитель // Известия Оренбургского государственного аграрного университета. – 2017; 3: 212–215.
  14. Kurtz D.M., Travlos G.S. The Clinical Chemistry of Laboratory Animals 3 Edition Edited. 2018. 1162 p.

You may be interested

NPO "Home of Pharmacy"

188663, Russia, Leningradskaya oblast', Vsevolozhskij rajon, g.p. Kuz'molovskij, Zavodskaya ulica, 3-245

© 2020 ООО «ИД «Русский врач»