Histological Evaluation Of Joint Pathology In Various Models Of Chronic Arthritis In Rats

Muzhikyan A.А.(1), Shekunova E.V.(1,2), Kashkin V.A.(1,2), Makarova M.N.(1), Makarov V.G.(1) 1 -“Institute of experimental pharmacology”, b. 245, 3, st. Zavodskaya, Kuzmolovskiy, Vsevolozhskiy district, Leningrad region, 188663, Russia, Leningrad region; 2- Valdman Institute of Pharmacology, Pavlov First St. Petersburg State Medical University, 6-8, L’va Tolstogo str., Saint Petersburg, 197022, Russia e-mail: [email protected]

DOI: 10.29296/2618723X-2018-01-04
Keywords: rheumatoid arthritis rat

For citation:

Muzhikyan A.A., Shekunova E.V., Kashkin V.A., Makarova M.N., Makarov V.G. Histological Evaluation Of Joint Pathology In Various Models Of Chronic Arthritis In Rats. Laboratory Animals for Science. 2018; 1. https://doi.org/10.29296/2618723X-2018-01-04


The aim of this work was to test the histological criteria for assessing changes in joints and to conduct a comparative analysis of two experimental models of chronic arthritis in the rat. The basis was taken method of assessment of cartilage by Malkin, which was originally developed in the 1950s to describe the cartilage in osteoarthritis is not inflammatory arthritis. It has been shown that the administration of complete Freund's adjuvant simulates the condition of rheumatoid arthritis with a significant increase in pannus, marked degenerative changes in cartilaginous tissue, osteolysis, fibrotic ankylosis of the joints and led to the spread of the pathological process to the joints removed from the place of CFA injection. In contrast, administration of carrageenan contributes to development of chronic inflammatory arthritis, lacking immunological component with inflammatory of the periarticular space and a slight damage to the cartilaginous tissue of the joints, only near the injection site of carrageenan. These observations should be considered in preclinical studies the specific pharmacological activity of innovative drugs with antirheumatoid, anti-inflammatory and/or immunomodulating properties.


  1. Scott D. L., Wolfe F., Huizinga T. W. Rheumatoid arthritis. Lancet. 2010. T. 376; 9746: 1094–108.
  2. Zinchuk I. Yu., Amirdzhanova V. N. Social`noe bremya revmatoidnogo artrita. Nauchno-prakticheskaya revmatologiya. 2014. T. 52; 3: 331–5.
  3. Joseph A., Brasington R., Kahl L., Ranganathan P., Cheng T. P., Atkinson J. Immunologic rheumatic disorders. J. Allergy Clin. Immunol. 2010. T. 125; 2. Suppl 2: 204–15.
  4. Hansra P., Moran E. L., Fornasier V. L., Bogoch E. R. Carrageenan-induced arthritis in the rat. Inflammation. 2000. T. 24; 2: 141–55.
  5. Hegen M., Sun L., Uozumi N., Kume K., Goad M. E., Nickerson-Nutter C. L., Shimizu T., Clark J. D. Cytosolic phospholipase A2alpha-deficient mice are resistant to collagen-induced arthritis. J. Exp. Med. 2003. T. 197, 10: 1297–302.
  6. Vinegar R., Truax J. F., Selph J. L., Johnston P. R., Venable A. L., McKenzie K. K. Pathway to carrageenan-induced inflammation in the hind limb of the rat. Fed Proc. 1987. T. 46, 1: 118–26.
  7. Bartlett R. R., Schleyerbach R. Immunopharmacological profile of a novel isoxazol derivative, HWA 486, with potential antirheumatic activity--I. Disease modifying action on adjuvant arthritis of the rat. Int. J. Immunopharmacol. 1985. T. 7, 1: 7–18.
  8. Blackham A., Burns J. W., Farmer J. B., Radziwonik H., Westwick J. An X-ray analysis of adjuvant arthritis in the rat. The effect of prednisolone and indomethacin. Agents Actions. 1977. T. 7, 1: 145–51.
  9. McCaffrey G., Staatz W.D., Sanchez-Covarrubias L., Finch J. D., Demarco K., Laracuente M. L., Ronaldson P. T., Davis T. P. P-glycoprotein trafficking at the blood-brain barrier altered by peripheral inflammatory hyperalgesia. J Neurochem. 2012. T. 122, № 5: 962–75.
  10. Hegen M., Keith J. C., Jr., Collins M., Nickerson-Nutter C. L. Utility of animal models for identification of potential therapeutics for rheumatoid arthritis. Ann. Rheum. Dis. 2008. T. 67, 11: 1505–15.
  11. Chang T. K., Huang C. H., Huang C. H., Chen H. C., Cheng C. K. The influence of long-term treadmill exercise on bone mass and articular cartilage in ovariectomized rats. BMC. Musculoskelet. Disord. 2010. T. 11: 185.
  12. Mankin H. J., Dorfman H., Lippiello L., Zarins A. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data. J Bone Joint Surg Am. 1971. T. 53; 3: 523–37.
  13. Whitehouse M. W. Adjuvant arthritis 50 years on: The impact of the 1956 article by C. M. Pearson, ‘Development of arthritis, periarthritis and periostitis in rats given adjuvants’. Inflamm. Res. 2007. T. 56% 4: 133–8.
  14. Bendele A., McComb J., Gould T., McAbee T., Sennello G., Chlipala E., Guy M. Animal models of arthritis: relevance to human disease. Toxicol. Pathol. 1999. T. 27; 1: 134–42.
  15. Fitzpatrick L. R., Green C., Frauenhoffer E. E., French K. J., Zhuang Y., Maines L. W., Upson J. J., Paul E., Donahue H., Mosher T. J., Smith C. D. Attenuation of arthritis in rodents by a novel orally-available inhibitor of sphingosine kinase. Inflammopharmacology. 2011. T. 19; 2: 75–87.

You may be interested