Technique Of Dissection And Extracting Organs Of Laboratory Animals. Message 1 (Rats)

K. Koptyaeva, researcher, pathomorphologist, A. Muzhikyan, PhD veterinary medicine, senior researcher, Head of the Laboratory of Histology and Pathomorphology, Ya.Gushchin, researcher, pathomorphologist, E. Belyaeva, researcher, pathomorphologist, M. Makarova, Director, V. Makarov, Doctor of Medicine, prof., Deputy dir. of science JSC «Research-and-manufacturing company «Houm оf Pharmacy», 188663, Russia, Leningradskiy region, Vsevolozhskiy district, Kuzmolovskiy, st. Zavodskaya, 3. b. 245 Е-mail: koptyaeva.ke@doclinika.ru

Abstract

Post-mortem examination of animals (necropsy, autopsy) is a diagnostic procedure aimed at finding out the cause of death of an animal, as well as determining the spectrum of macroscopically visible changes in organs and tissues of cadaver. In scientific and research practice, one of the key stages of the experiment and at the same time a humane experimental point often is euthanasia of animals with subsequent pathoanatomical and histological examination of organs. From the point of view of the quality and reliability the obtained data, an important step is the standardization of the procedure to dissection and extracting organs of laboratory animals. A series of articles about the carrying out of necropsy of laboratory animals will highlight the main stages of autopsy. In this work, a technique to dissection and extracting organs of rats is described.

References

  1. Muzhikyan A.A., Makarova M.N., Gushhin Ya.A. Osobennosti patologoanatomicheskogo issledovaniya gruppy e`ksperimental`nyh zhivotnyh. SPb: Mezhdunarodnyy vestnik veterinarii. 2014; 1: 75–80.
  2. Rybakova A.V., Makarova M.N. Metody e`vtanazii laboratornyh zhivotnyh v sootvetstvii s Evropeyskoy direktivoy 2010/63. Mezhdunarodnyy vestnik veterinarii. 2015; 2: 96–107.
  3. Fiette L1, Slaoui M. Necropsy and Sampling Procedures in Rodents // Drug Safety Evaluation: Methods and Protocols, Methods in Molecular Biology. 2011. Vol. 691: 39–56. doi :10.1007/978-1-60761-849-2_3
  4. McInnes E. (Ed.). Pathology for Toxicologists: Principles and Practices of Laboratory Animal Pathology for Study Personnel. Chichester: John Wiley & Sons. 2017: 25–6.
  5. Parkinson C. et al. Diagnostic necropsy and selected tissue and sample collection in rats and mice. Journal of Visualized Experiment. 2011, 54: 2–7. doi: 10.3791/2966
  6. Revised guides for organ sampling and trimming in rats and mice – Part 1 // Experimental and Toxicologic Pathology. 2003. Vol. 55: 91–106. doi: 10.1078/0940-2993-00311
  7. Scudamore C. L. (Ed.). A Practical Guide to the Histology of the Mouse. Chichester: John Wiley & Sons. 2014: 17–20.
  8. Suckow M.A., Stevens K.A., Wilson R.P. (Eds.). The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents. London: Academic Press; Elsevier. 2012: 135–6.

You may be interested