Barashkova S.V., Zhuravky S.G., Alekseenko S.I. Тhe Method Of Intravital Diagnosis Of Ciliary Activity Of Columnar Ciliated Epithelium Of The Nasal Mucosa In Laboratory Rats. Laboratory Animals for Science. 2018; 1. https://doi.org/10.29296/2618723X-2018-01-07
The results of a comparative study of the intravital cytological material of the columnar ciliated epithelium of the nasal cavity from intact adult rats of the Wistar (220-250 g) contained in the conventional type vivarium (n = 2) and SPF category (n = 2) are presented. An intact picture of cells of the columnar ciliated epithelium is noted in the material of SPF animals. A subacute inflammation and persistence of bacterial infection in animals of the conventional vivarium were observed. In this case, there were morphofunctional signs of degeneration of the ciliary apparatus: shortening, asynchronism and incomplete cycle of beating of the cilia. The best quality of the cytological material for in-vivo biopsy was obtained by using the combined cervical brushes «Cervex-Brush Combi» made of a polymer material modified by trimming the bristles of the central brush.