The relationship of amyloidogenesis and taupathy with clinical outcomes of cerebral ischemia. Experimental research

Original article
УДК 616-003.821
DOI: 10.57034/2618723X-2023-02-02

D.I. Pozdnyakov*,
Associate professor of the Department of Pharmacology with a course of clinical pharmacology,
https://orcid.org/0000-0002-5595-8182

Pyatigorsk Medical and Pharmaceutical Institute — branch of the Volga State Medical University,
357532, Russia Stavropol Territory, Pyatigorsk, Kalinin ave., 11.

* E-mail: [email protected]

Keywords: brain ischemia beta-amyloid tau protein cognitive deficit neurological deficit sensorimotor deficit
Acknowledgements

The study was performed without external funding.

For citation:

Pozdnyakov D. I. The relationship of amyloidogenesis and taupathy with clinical outcomes of cerebral ischemia. Experimental research. Laboratory Animals for Science. 2023; 2. https://doi.org/10.57034/2618723X-2023-02-02

Abstract

Currently, it has been established that increased formation of β-amyloid and tau protein play a significant role in the pathogenesis of cerebral ischemia. At the same time, these molecules can act as prognostic markers of the severity of cerebral ischemia. The aim of the study. To evaluate the peculiarities of changes in the concentration of β-amyloid and tau protein in blood serum and cerebrospinal fluid in rats with experimental subtotal cerebral ischemia, as well as to establish their relationship with the severity of post-ischemic clinical changes. Materials and methods. Subtotal cerebral ischemia was modeled by simultaneous occlusion of common carotid arteries in Wistar rats. The study assessed changes in neurological, sensorimotor and cognitive deficits. The content of β-amyloid and tau protein was assessed in blood serum and cerebrospinal fluid on the 1st, 3rd, 5th and 7th days of ischemia. During the analysis, species-specific kits for solid-phase enzyme immunoassay were used. Results. It was found that in animals with subtotal cerebral ischemia, as the duration of the ischemic period increases from day 1 to day 7, the concentration of β-amyloid and tau protein in both blood serum and cerebrospinal fluid progressively increases. It is worth noting that these changes strongly correlated with changes in the clinical manifestations of cerebral ischemia — neurological, sensorimotor and cognitive deficits.

Conflict of interest

The authors declare no conflict of interest.

  1. Директива 2010/63/EU Европейского парламента и Совета Европейского союза по охране животных, используемых в научных целях / пер. с англ. Под ред. М.С. Красильщиковой, И.В. Белозерцевой. Санкт-Петербург, 2012. 48 с. [Direktiva 2010/63/EU Yevropeyskogo Parlamenta i Soveta Yevropeyskogo Soyuza po okhrane zhivotnykh, ispol’zuyemykh v nauchnykh tselyakh / transl. from English. Ed. M.S. Krasilshchikova, I.V. Belozertseva. St. Petersburg, 2012. 48 p. (In Russ.)].

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Received: 2023-03-18
Reviewed: 2023-05-12
Accepted for publication: 2023-05-23

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