Morphofunctional and histochemical features of the structure of the left lung of laboratory outbred albino rats

DOI: 10.29296/2618723X-2021-04-02

S.V. Zinoviev, Senior Researcher, Candidate of Medical Sciences, ORCID 0000-0001-6022-6130,
N.G. Plekhova, Head of the Central Research Laboratory, Doctor of Biological Sciences, ORCID 0000-0002-8701-7213,
I.V. Radkov, Junior Researcher, ORCID 0000-0001-6022-6130
Pacific State Medical University of the Ministry of Health of the Russian Federation

Е-mail: [email protected]


Keywords: histochemistry rat left lung arterioles venules

For citation:

Zinoviev S.V., Plekhova N.G., Radkov I.V. Morphofunctional and histochemical features of the structure of the left lung of laboratory outbred albino rats. Laboratory Animals for Science. 2021; 4. https://doi.org/10.29296/2618723X-2021-04-02

Abstract

It is necessary to search for a method with which it is possible to extrapolate the indicators of the metabolic process to the organo-tissue level of lung organization in albino rats. Purpose of the study: to reveal the structural features of the left lung (LL) of outbred albino rats on the basis of histochemical analysis data. The experiment was carried out on sexually mature male Wistar rats (200–250 gr.). For the histochemical detection of calcium cations, the method of tissue staining with alizarin red C (ARC) and boric acid (BA) was used. After anesthesia and vivisection, the whole LL, extracted from the chest, was placed for 48 hours in a 5% ARC solution in 960 ethyl alcohol. Then the pieces of tissue were embedded in paraffin using chloroform solutions for dehydration. The sections obtained on a microtome were dewaxed in xylene and stained with an alcoholic solution of BA (5 min), after which they were examined under a fluorescent microscope.

Results. Supravital staining with alcoholic ARC solution in the stroma and on the surface of the alveoli of rat lungs reveals cells containing in the cytoplasm from 1 to 7 optically dense granules up to 3 μm in size. In the tissues of the lungs, basement membranes, cells containing structures with diol groups, are noted, and nuclei and nucleoli are visualized in them. In the middle sheath of the proximal generations and the caudal vein itself, fluorescent cardiomyocytes were observed, near which luminous thick connective tissue fibers were located. The distal veins of the respiratory parenchyma did not contain cardiomyocytes. The fluorescence of the bronchi stained with ARC and BA in the red range indicated an increased content of sugars and tyrosines, while the bright glow of the connective tissue fibers of the mucous membrane and basement membranes in the yellow-green range indicated a low content of tyrosine and sugars.

Thus, the method of processing LL tissues with staining of its tissues with ARC and BA allows for a clearer visualization of the structures of the bronchi, veins and arteries compared to the routine histological one.

Full text avaliable in Russain only 

Author’s Contribution

Zinoviev S.V. – concept or design of the work; collection, analysis or interpretation of work results; writing text.

Plekhova N.G. – approval of the final version of the article for publication; agreement to be responsible for all aspects of the work, due study and resolution of issues related to the reliability of data or the integrity of all parts of the article.

Radkov I.V. – collection, analysis or interpretation of work results.

 

Conflict of interest

The authors declare no conflicts of interest.

References

  1. Матичин А.А., Кательникова А.Е., Крышень К.Л. Особенности отбора бронхоальвеолярного лаважа у лабораторных животных. //Лабораторные животные для научных исследований. 2019; 4: 6. [Matichin A.A., Katel'nikova A.E., Kryshen K.L. Specific techniques of bronchoalveolar lavage collecting from laboratory animals. //Laboratornye zhivotnye dlya nauchnyh issledovanij. 2019. 4: 6. (In Russ )] doi.org/10.29296/2618723X-2019-04-06.
  2. Wright J.L., Cosio M., Churg A. Animal models of chronic obstructive pulmonary disease. //Am J Physiol Lung Cell Mol Physiol. 2008 ; 295(1): 1–15. doi: 10.1152/ajplung.90200.2008.
  3. Королев И.Б., Котельников В.Н., Кириллов О.И., Антонюк М.В. Некоторые аспекты вегетативной дисфункции при экспериментальной артериальной гипертензии у крыс//Тихоокеанский медицинский журнал. 2010; 1 (39): 18-20.[ Korolev I.B., Kotel'nikov V.N., Kirillov O.I., Antonyuk M.V. Some aspects of autonomic dysfunction in experimental arterial hypertension in rats //Tihookeanskij medicinskij zhurnal. 2010; 1 (39): 18-20. (In Russ )]
  4. Зиновьев С.В., Целуйко С.С., Селиверстов С.С., Горбунов М.М. Cтрессорное легкое крыс как экспериментальная модель легочной гипертензии и гиперемии. //Бюллетень физиологии и патологии дыхания. 2018. 67: 102-110. [Zinov'ev S.V., Celujko S.S., Seliverstov S.S., Gorbunov M.M. Stressing lung of rats as an experimental model of pulmonary hypertension and hyperemia Byulleten' fiziologii i patologii dyhaniya. 2018; 67: 102-110. (In Russ )].
  5. Петренко В.М. Aнатомия легких у белой крысы. // Международный журнал прикладных и фундаментальных исследований. 2013; 10-3: 414- 417; [Petrenko V.M. Anatomy of the lungs in a white rat. Mezhdunarodnyj zhurnal prikladnyh i fundamental'nyh issledovanij. 2013. № 10-3. С. 414-.417 (In Russ )]
  6. Kummer W. Pulmonary vascular innervation and its role in responses to hypoxia size matters // Proc. Am. Thorac. Soc. 2011; 8 (6):471–476. (In Russ )]. doi: 10.1513/pats.201101-013MW.
  7. Чумасов Е.И., Ворончихин П.А., Коржевский Д.Э. Эфферентная иннервация сосудов и бронхов легкого крысы (иммуногистохимическое исследование). Морфология. 2012; 142(4): 49-53. [Chumasov E. I., Voronchikhin P. A., Korzhevskii D. É. Efferent innervation of pulmonary blood vessels and bronchi in rat (an immunohistochemical study). //Morfologiia. 2012;142(4):49-53. (In Russ )].
  8. Uggere T.A., Abreu G.R., Sampaio K.N., Cabral A.M., Bissoli N.S. The cardiopulmonary reflexes of spontaneously hypertensive rats are normalized after regression of left ventricular hypertrophy and hypertension.// Braz J Med Biol Res. 2000 ; 33(5):589-94. doi: 10.1590/s0100-879x2000000500014.
  9. Зиновьев С.В. Гистохимическая характеристика венозного русла респираторного отдела легких экспериментальных животных, подвергнутых хроническому переохлаждению, после введения в организм дигидрокверцетина. Бюллетень физиологии и патологии дыхания. 2012; 45: 57-61. [Zinov'ev S.V., Histochemical characteristic of the vein channel of lungs respiratory part of experimental animals subjected to chronic hypothermia after dihydroquercetin intake Byulleten' fiziologii i patologii dyhaniya. 2012; 45: 57-61. (In Russ )].
  10. Chinoy N.J., Sanjeevan A.G. On the specificity of alcoholic acidic silver nitrate reagent for the histochemical localization of ascorbic acid. //Histochemistry. 1978; 56: 275–282. doi.org/10.1007/BF00495989
  11. Hosoyamada Y., Ichimura K., Koizumi K., Sakai T. Structural organization of pulmonary veins in the rat lung, with special emphasis on the musculature consisting of cardiac and smooth muscles. //Anat Sci Int. 2010; 85: 152–159 . doi.org/10.1007/s12565-009-0071-9
  12. Lacina, K., Skládal, P. James T.D. Boronic acids for sensing and other applications – a mini-review of papers published in 2013. //Chemistry Central Journal. 2014; 8: 60 (). Doi: 10.1186/s13065-014-0060-5
  13. Mako, T. L., Racicot, J. M., Levine, M. Supramolecular Luminescent Sensors. //Chem Rev. 2019; 119(1):322-477 doi: 0.1021/acs.chemrev.8b00260.

You may be interested