DOI:
10.29296/2618723X-2018-04-06
D. Kargopoltseva(1), K. Kryshen(1), A. Katelnicova(1), Ya. Gushchin(2), M. Makarova(2), V. Makarov(1) 1-Institute of pre-clinical studies 188663, Leningradskiy region, Vsevolozhskiy district, Kuzmolovskiy, 3, Zavodskaya st., b. 245, Russia; 2-Scientific-Production Organization «Houm оf Pharmacy» 188663, Leningradskiy region, Vsevolozhskiy district, Kuzmolovskiy, 3, Zavodskaya st., b. 245, Russia E-mail: [email protected]
Keywords:
formalin
DNHB
skin sensitization
Magnusson and Kligman test
guinea pigs
For citation:
Kargopoltseva D., Kryshen K., Katelnicova A., Gushchin Ya., Makarova M., Makarov V. Experience Of Magnusson And Kligman Guinea Pigs Maximization Test In Studies Of The Sensitizing Potential Of Chemical Substances. Laboratory Animals for Science. 2018; 4. https://doi.org/10.29296/2618723X-2018-04-06
Abstract
Summary. According to OECD Test No: 406, the necessary research for drug registration for skin application is the study of the sensitizing potential in the Magnusson and Kligman maximization test (adjuvant test) and Bühler (non-adjuvant test). The complete Freund’s adjuvant (PAF) is used as an adjuvant in the maximization test of Magunsson and Kligman. The complete Freund’s adjuvant is nonspecifically enhances the immune response to foreign antigens and enhances the immune response in the first stage of sensitization. The Magnusson and Kligman maximization test is used in most cases in the European Union, according to literary sources. This paper describes the procedure of performed this test, as well as an analysis of the sensitizing potential of chemicals using the example of formalin and dinitrochlorobenzene, which can be used as a positive control when conducting further research. This study included 3 stages: the stage of sensitization 1 with the intradermal injection of the test articles and PAF; stages of sensitization 2 and challenge with skin applications. The concentration of test articles used for each induction exposure was well-tolerated systemically and was the highest to cause mild-to-moderate skin irritation.The concentration used for the challenge stage was the highest non-irritant dose. Evaluation of the skin reaction was performed after 48 and 72 hours after the application of the test articles on the Magnusson-Kligman scale. According to the results of the study, it was found that a more intense skin reaction was detected when using DNHB, which registered visually and microscopically.
References
-
OECD. Skin Sensitisation. OECD Guideline for the Testing of Chemicals. 1992; 406.
-
Sargent E.V. Occupational toxicology testing. Comprehensive toxicology. 2018. Vol. 9: 44–63.
-
Magnusson B. and Kligman A.M. Allergic Contact Dermatitis in the Guinea Pig. Charles G. Thomas; Springfield, Illinois. 1970.
-
Metody ispytaniya po vozdeystviyu himicheskoy produkcii na organizm cheloveka. Ispytaniya po ocenke kozhnoy sensibilizacii: GOST 32375-2013. Vved. 01-08-2014. M.: Izd-vo standartov, 2014.
-
Magnusson B. and Kligman A.M. The identification of contact allergens by animal assay. The guinea pig maximisation test. Journal of Investigative Dermatology. Vol. 52: 268–76.
-
Maurer T. The maximization test for skin sensitization potential – updating the standard protocol and validation of a modified protocol. Food and Chemical Toxicology. 1989; 27 (12): 807–11.
-
Klaus E. Andersen The guinea pig maximization test – with a Multiple dose design. Acta derm venereol. 1995; 75: 463–9.
-
Shillaher O., Bell M. Guinea pig maximization test for skin sensitization the use of fewer test animals. Arch Toxicol. 1989. Vol. 63: 283–8.
-
Gushhin Ya.A., Muzhikyan A.A. Vliyanie fiksiruyushhih zhidkostey na mikroskopicheskuyu strukturu organov melkih laboratornyh zhivotnyh. Mezhdunarodnyy vestnik veterinarii.2014; 3: 88–95.
-
Muzhikyan A.A., Makarova M.N., Gushhin Ya.A. Osobennosti gistologicheskoy obrabotki organov i tkaney laboratornyh zhivotnyh. Mezhdunarodnyy vestnik veterinarii. 2014; 2: 103–9.