Impact Assessment оf Potential Risks From Enviromental Temperature Decrising оf Laboratory Animals (Mice, Rats, Guinea Pigs) Outside the Recommended Range for Main Biological Parameters

D.R. Kargopoltceva,   ORCID  0000-0002-9944-5223, 
M.N. Makarova,   ORCID  0000-0003-3176-6386, 
A.A. Zueva,
   ORCID 0000-0002-2996-0748, 
K.Yu. Kildibekov, ORCID 0000-0002-0696-6987 

«Institute of Preclinical Research» Ltd. 188663, Russia, Leningrad oblast, Vsevolozhskiy district, Kuzmolovskiy t.s., Zavodskaya st. 3-245

Е-mail: kargopoltceva.dr@doclinika.ru

Abstract

Risks are the possibility of an adverse situation attracts various losses. There are risks in all spheres of activity including the work of vivariums and sources. It is important to observe the necessary environmental conditionals (room temperature, air humidity and air exchange) and light cycle. It may be deviations from the established standards for these microclimate indicators that, in turn, might severely effects on animal health and trigger inaccurate study results in case emergency situations in the vivarium. In previous work study was conduct to assess the potential risks resulting from elevation of environmental temperature of laboratory animals (mice, rats and guinea pigs). It was found, that an environmental temperature increase by 4°C above the upper limit of the recommended ranges didn’t effect on vital indicators of laboratory animals. In current study we researched risks emerging by decrease of the environmental temperature 3°C above lower recommended range influence main health indicators like: general animal state, body weight, hematological and blood biochemical parameters and weight of internal organs to body weight ratio. Mice, rats and guinea pigs were housed at the temperature: 19, 18 and 17°C for week. Other animals were housed for 3 weeks at the initial temperature 19°C with gradual lowering by 1°C weekly until 17°C. The study found that the small lowering of environmental temperature (between 19 and 17°C) didn’t effect on animal vital signs and risks in case of emergency situations related to the provision of the recommended content temperature are minimal.

Full text avaliable in Russain only

References

  1. Каргопольцева Д.Р., Крышень К.Л., Кательникова А.Е., Макарова М.Н., Макаров В.Г., Зуева А.А., Матичин А.А., Гайдай Д.С. Оценка потенциальных рисков, возникающих при повышении температуры содержания лабораторных животных (мыши, крысы, морские свинки) за пределы рекомендованных диапазонов по влиянию на основные биологические показатели // Лабораторные животные для научных исследований. 2019. №3. DOI: 10.29296/2618723X-2019-03-09 [Kargopol'ceva D.R., Kryshen' K.L., Katel'nikova A.E., Makarova M.N., Makarov V.G., Zueva A.A., Matichin A.A., Gajdaj D.S. Ocenka potencial'nyh riskov, voznikayushchih pri povyshenii temperatury soderzhaniya laboratornyh zhivotnyh (myshi, krysy, morskie svinki) za predely rekomendovannyh diapazonov po vliyaniyu na osnovnye biologicheskie pokazateli // Laboratornye zhivotnye dlya nauchnyh issledovanij. 2019. №3. (In Russ.)].
  2. Мартынов Н.Н., Санникова О.А. Влияние длительной холодовой адаптации на поведение крыс Wistar // Лабораторные животные для медико-биологических и биотехнологических исследований: тезисы конф. (Москва, 20 – 22 ноября 1990 г.) – Москва, 1990. – С. 51. [Martynov N.N., Sannikova O.A. Vliyanie ditel'noj holodovoj adaptacii na povedenie krys Wistar // Laboratornye zhivotnye dlya mediko-biologicheskih i biotekhnologicheskih issledovanij: tezisy konf. (Moskva, 20 – 22 noyabrya 1990 g.) – Moskva, 1990. – P. 51. (In Russ.)].
  3. Ferreira M. et al. Electric countershock and cold stress effects on liver and adrenal gland // Clinics. 2010. Vol. 65(3): 291-296. DOI: 10.1590/S1807-59322010000300009.
  4. Kulkarni M. P., Juvekar A. R. Attenuation of acute and chronic restraint stress-induced perturbations in experimental animals by Nelumbo nucifera Gaertn // Indian journal of pharmaceutical sciences. 2008. Vol. 70 (3): 327-332. DOI: 10.4103/0250-474X.42982.

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