Methods of Visualization and Analysis of The Microcirculatory State in Veterinary and Experimental Practices

Vasutina (1, 4), N. Pechnikova(1–3), Ya. Toropova(1)

1 - Federal Amlazov North-West Medical Research Centre, 194156, Saint-Petersburg, Russia st. Parkhomenko Avenue 15;
2 - Saint-Petersburg State Chemical-Pharmaceutical University, 197022, Saint-Petersburg, Russia st. Professor Popov, 4;
3 - Zoological Institute, Russian Academy of Sciences 199034, Saint-Petersburg, Russia st. University Embankment 1;
4 - Federal State Budgetary Educational Institution of Higher Education "Saint-Petersburg State Academy of Veterinary Medicine"
196084, Russia, Saint-Petersburg, st. Chernigovskaya, 5


In veterinary medicine and experimental studies there are still questions about the quality of microcirculation and the condition of microcirculation vessels. Normal functioning of organs as such and of the organism as a whole is determined by the state of regulatory systems and individual links of the microcirculatory channel. Currently, the visualization of the microcirculation channel is carried out by invasive and non-invasive methods. However, preference is given to indirect methods of diagnosing the condition of vessels based on the analysis of metabolic processes in the body. This review provides information on the most common methods of visualization and diagnostics of microvascular channel condition in experimental and veterinary practices. Literature review was carried out with the help of the MEDLINE search system, starting from January 2008, the following keywords were used: microcirculation, visualization of blood vessels, analysis of vessels. The data obtained showed that in veterinary practice, non-invasive methods are usually used to evaluate the microvascular channel. The most accessible and informative methods are spectrophotometry, rheovasography, laser Doppler flowmetry (including ultrasonic biomicroscopy), as well as microwave radiothermometry, X-ray contrast honey, vasography, venography and lymphography. In addition, magnetic resonance angiography, pulse oximetry and computerized capillaroscopy are informative methods for vascular diagnostics. In turn, various optical techniques (luminescent, ultraviolet, interference, phase-contrast microscopes), optical quantum generators, achievements of electronics (electron and television microscopes), new types of sensors and amplifiers, have existing limitations in practical application (do not allow to analyze the functional aspect in the study of microcirculation links), as a result of which knowledge in this field is still observed mainly at the level of morphology of individual components. Intravital fluorescence digital microscopy becomes the most attractive method for practical purposes to assess the state of blood and lymphatic flow.

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