Technique of Dissection and Extracting Organs of Laboratory Animals. Message 4: Guinea Pig, Gerbil, Degu

K.E. Koptyaeva, Ya.A. Guschin, E.V. Belyaeva, M.N. Makarova, V.G. Makarov

Research-and-manufacturing company «Home оf Pharmacy»
188663, Russia, Leningradskiy region, Vsevolozhskiy district, Kuzmolovskiy, b. 245, 3, st. Zavodskaya,
Е-mail: koptyaeva.ke@doclinika.ru

Abstract

The fourth message from the series of articles on the methodology of necropsy and extraction organs of laboratory animals, describing in detail and illustrating the technique of sequential and complete autopsy and extraction of organs of laboratory guinea pigs, gerbils and degu. Guinea pigs are often chosen to study the allergenic properties of drugs and to determine the pharmacological activity, for example, in experiments that model hypercholesterolemia, airway hyperresponsiveness, etc. The high susceptibility of gerbils makes it possible to be a model for various infectious diseases. Also on them examine the effect of drugs on the level of steroid hormones and cholesterol. Degus that predisposed to the development of diabetes mellitus are a good model for studying this disease. In addition to this, on them conduct studies of cataracts, atherosclerosis, and Alzheimer's disease. This article demonstrates the procedure for necropsy of laboratory guinea pigs, gerbils, and degu. It describes the process of preparing an animal for an autopsy, conducting a primary examination of a corpse after euthanasia for the presence of external changes and injuries, fixing the corpse on the dissecting table, necropsy and examining the internal cavities of the body, as well as examining the superficial lymph nodes. Possible methods for the extraction of organs of the oral cavity (including extraction of the tongue, pharynx), all organs of the thoracic, abdominal and pelvic cavities are described and illustrated. The topographic location of some organs of the thoracic, abdominal and pelvic cavities is illustrated. Prescribed two ways to extract the brain, the method of separation of the chest bone for histological examination of the bone marrow and upper jaw for histological examination of the nasal passages. The procedure for preparing the spinal cord to formalin fixation without removing it from the spinal canal is described. Eye extraction with the lacrimal gland and eyelids, extraction of muscle and adjacent peripheral nerves is described. The methods of extraction and the sequence of actions performed, described in this article, minimize the damage of the organs being removed and prevent their contamination in order to prevent the occurrence of certain artifacts detected by subsequent histological examination. Conclusions are presented at the end of the article.

Full text available only in Russian

References

  1. Koptyaeva K.E., Muzhikyan A.A., Gushhin Ya.A., Belyaeva E.V., Makarova M.N., Makarov V.G. Metodika vskry`tiya i izvlecheniya organov laboratorny`x zhivotny`x. Soobshhenie 1: kry`sa. Laboratorny`e zhivotny`e dlya nauchny`x issledovanij. 2018. N2. C. 71-92. doi: 10.29296/ 10.29296/2618723X-2018-02-08.
  2. Koptyaeva K.E., Muzhikyan A.A., Gushhin Ya.A., Belyaeva E.V., Makarova M.N., Makarov V.G. Metodika vskry`tiya i izvlecheniya organov laboratorny`x zhivotny`x. Soobshhenie 2: my`sh`. Laboratorny`e zhivotny`e dlya nauchny`x issledovanij. 2018. N4. C. 50-73. doi: 10.29296/2618723X-2018-04-05.
  3. Koptyaeva K.E., Muzhikyan A.A., Gushhin Ya.A., Belyaeva E.V., Makarova M.N., Makarov V.G. Metodika vskry`tiya i izvlecheniya organov laboratorny`x zhivotny`x. Soobshhenie 3: xomyachok. Laboratorny`e zhivotny`e dlya nauchny`x issledovanij. 2019. N1. C. 50-73. doi: 10.29296/2618723X-2019-01-02.
  4. Muzhikyan A.A., Makarova M.N., Gushhin Ya.A. Osobennosti patologoanatomicheskogo issledovaniya gruppy` e`ksperimental`ny`x zhivotny`x. SPb.: Mezhdunarodny`j vestnik veterinarii. 2014. N1. S. 75-80.
  5. Ry`bakova A.V. Metody` e`vtanazii laboratorny`x zhivotny`x v sootvetstvii s evropejskoj direktivoj 2010/63 / A.V. Ry`bakova, M.N. Makarova // Mezhdunarodny`j vestnik veterinarii. 2015. N2. S. 96-107.
  6. Fiette L., Slaoui M. Necropsy and Sampling Procedures in Rodents // Drug Safety Evaluation: Methods and Protocols, Methods in Molecular Biology. 2011. Vol. 691. P. 39-56. doi :10.1007/978-1-60761-849-2_3
  7. McInnes E. (Ed.). Pathology for Toxicologists: Principles and Practices of Laboratory Animal Pathology for Study Personnel. Chichester: John Wiley & Sons. 2017. P. 25-26.
  8. Parkinson C. et al. Diagnostic necropsy and selected tissue and sample collection in rats and mice // Journal of Visualized Experiment. 2011. N54. P. 2-7. doi: 10.3791/2966
  9. Revised guides for organ sampling and trimming in rats and mice – Part 1 // Experimental and Toxicologic Pathology. 2003. Vol. 55. P. 91-106. doi: 10.1078/0940-2993-00311
  10. Reznik G., Schuller, Hildegard M., Mohr U. Clinical anatomy of the European hamster, Cricetus cricetus, L. Bethesda, Maryland. 1978. URL: digital.library.unt.edu/ark:/67531/metadc28308 (accessed: 09.01.2019)
  11. Scudamore C. L. (Ed.). A Practical Guide to the Histology of the Mouse. Chichester: John Wiley & Sons. 2014. P. 17-20.
  12. Suckow M.A., Stevens K.A., Wilson R.P. (Eds.). The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents. London: Academic Press; Elsevier. 2012. P. 135-136.

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