Immunoadhesive Mechanisms Of Phytoadaptogen Preventive Action On Spontaneous Hepatocancerogenesis

E.V. Bocharov(1) , Candidate of Medical Sciences, Senior Researcher Lab. immunopharmacology, R.V. Karpova(1) , Candidate of Biological Sciences, Senior Researcher Lab. immunopharmacology, O.A. Bocharova(1) , Doctor of Biological Sciences, Professor, Head of the Lab. immunopharmacology, V.G. Kucheryanu(2) ,Doctor of Medical Sciences, Chief researcher lab. general pathology of the nervous system, I.V. Kazeev(1) , Candidate of Technical Sciences, Senior Researcher Lab. immunopharmacology, V.A. Misyurin(1) , Candidate of Biological Sciences, Senior Researcher, Laboratory of Experimental Diagnostics and Tumor Biotherapy, Yu.N. Soloviev(1) , Doctor of Medical Sciences, Professor, Chief Researcher of the Department of Pathological Anatomy of Human Tumors 1-N.N. Blokhin National Medical Research Center of oncology, Ministry of Health of Russia Russia, 115478, Moscow, 24 Kashirskoe shosse; 2-Institute of general pathology and pathophysiology Russia, 125315 Moscow, 8 Baltiyskaya St. Е-mail: preclin5_dep@iphar.ru

Abstract

Up-regulated leukocyte integrins expression on peripheral blood cells, reduced IL-6 and IL-10 serum levels accompanied by tumour’s lymphocyte infiltration and destruction using phytoagentogen complex (PAC) early ontogenesis preventive administration is essential for tumour incidence down-regulation, as well as the life-span and life quality increase of high-cancer animals. The preventive effect was carried out on the example of a PAC nontoxic natural immunomodulator, standardized by biological and chemical methods, and consisting of 40 hydro-alcoholic plant extracts components, including adaptogens (Panax ginseng, Eleutherococcus senticosus, Rhodiola rosea, etc.) as well as compounds of phenolic nature (flavonoids, triterpene glycosides, etc.). PAC has antimutagenic, antioxidant, immunomodulating properties. It was used as a 10% solution with drinking water during the first month of postnatal ontogenesis of CBA mice, including the period (5–15 days of life) corresponding to the completion of normal liver tissue differentiation. The CD11a and CD11b antigens expressions on peripheral blood cells were analyzed by indirect immunofluorescence reaction, serum cytokines IL-6 and IL-10 concentrations were determined by enzyme-linked immunosorbent assay as well as the liver tissue morphology were analyzed at the age of 4, 8, 22 months. Tumour incidence and volume were evaluated at the age of 8 and 22 months. Motor activity and physical status (body weight, coat state) were estimated in the ontogenesis. The average life-span and survival median was analyzed by the Kaplan-Meier method. Statistical analysis was performed with the program Statistica 6.0. PAC preventive administration revealed the long-term heterotypic adhesion molecules leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) up-regulated expression, providing the contact interactions of immune effectors and cancer cells. The events shown is able to enhance the anti-tumour activity of immune reactions including spontaneous hepatocarcinomas lymphocytes infiltration and destruction of tumour tissue. There was also a decrease in the serum level of the suppressor cytokines IL6 and IL10. As a result, the hereditary tumours incidence, their number and sizes were reduced as well as life-span and life quality of animals were improved.

References

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