The Practice Of Modeling Pyoinflammatory Wounds At The Immunosuppression

E. Pariyskaya, PhD, Associate Professor, L. Zakharova, PhD, Associate Professor, O. Orlova, PhD, Associate Professor, O. Rybalchenko, DSc, Professor, N. Golovanova, PhD, Associate Professor, I. Astratenkova, PhD, Associate Professor St. Petersburg State University 199034, Russian Federation, St. Petersburg, Universitetskaya nab., 7 Е-mail: nesh1764@mail.ru

Abstract

Summary. Treatment of purulent complications of soft tissues is one of the most common problems in medical practice presently. In this regard, there is a constant work on the improvement of methods of exposure and analysis of the effect of medicine on various models of purulent wounds of animals. The models presented in the literature cannot always successfully reproduced in practice. In this article, several variants of modeling the development of purulent-inflammatory process in skin wounds in rats are considered. At the first stage, a comparative analysis of the survival of rats under the influence of hydrocortisone and methotrexate was carried out to select the most effective immunosuppressant. Then, bacteria of two types tested as infecting microorganism wounds: Staphylococcus aureus 6, a representative of the normal microbiota of the skin, and Pseudomonas indica 23, an analogue of the most common type of pseudomonads, the causative agents of nosocomial infections. Infection of wounds was carried out using a homogeneous and mixed suspension of the two above-mentioned bacterial cultures, as well as monocultures in the form of biofilms. As a result of the research, the most optimal model for obtaining purulent wounds turned out to be a variant using immunosuppression with hydrocortisone. Exposure to hydrocortisone for seven days (25 mg / kg), in contrast to methotrexate, did not lead to animal mortality. In this case, a single injection of the mixed bacterial culture of S. aureus 6 and P. indica 23 caused the development of a purulent-inflammatory process within 48 hours. An increase in the diameter of the internal opening of the silicone fixator, in which the wound is located, made it possible to carry out a more precise planimetric assessment of damage and to observe visually the speed of marginal epithelialization.

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