Recommendations For Testing The Diabetogenic Activity Of Streptozotocin

M.А. Kovaleva(1), E.V. Shekunova(2), V.A. Kashkin(2), М.N. Makarova(1), V.G. Makarov(2) 1-JSC Research-and-manufacturing company «Houm оf Pharmacy», Leningradskiy region, Vsevolozhskiy district, Kuzmolovskiy, st. Zavodskaya, 3. b. 245, 188663, Russia; 2-CJSC «Institute of experimental pharmacology» Leningradskiy region, Vsevolozhskiy district, Kuzmolovskiy, st. Zavodskaya, 3. b. 245, 188663, Russia

Abstract

Currently streptozotocin (STZ) is the most common diabetogenic substance used to model experimental diabetes mellitus on different types of laboratory animals during last 50 years. It should be noted that there are no organizations on the territory of the Russian Federation that manufacture the substance of STZ. The of Russian scientific centers purchase STZ in foreign organizations. Despite the introduction of production standards around the world, depending on the manufacturer, the quality of STZ, including physicochemical and biological properties is very different. Also special attention is required by the conditions of transportation of STZ, which require compliance with the temperature conditions of storage and transport of substance specified by the manufacturer. To ensure the rational conduct of preclinical research and the principles of humane treatment of laboratory animals, it is recommended to get biological testing of each new pack of STZ in small rodents: rats and mice. Biological testing of STZ should also be executed for an already opened pack that was stored for more than 5 months after the first opening.

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