Тhe Method Of Intravital Diagnosis Of Ciliary Activity Of Columnar Ciliated Epithelium Of The Nasal Mucosa In Laboratory Rats

DOI: 10.29296/2618723X-2018-01-07

Barashkova S.V.(1,3), Zhuravky S.G.(2,3), Alekseenko S.I.(1,4) 1-Children’s City Hospital № 19 named after K.A. Rauhfus, b.8, Ligovsky Avenue, Saint Petersburg, 191036, Russia 2-Pavlov First Saint Petersburg State Medical University, b. 6-8, L. Tolstogo str., Saint Petersburg, 197022, Russia 3-Almazov National Medical Research Centre, b.2, Akkuratov str., Saint Petersburg, 197341, Russia 4-Mechnikov North-West State Medical University, Kirochnaja, 1, Saint Petersburg, 191015, Russia, е-mail: [email protected]

Keywords: ciliary apparatus phase contrast high-speed video imaging method native cytological material rats of SPF-status
For citation:

Barashkova S.V., Zhuravky S.G., Alekseenko S.I. Тhe Method Of Intravital Diagnosis Of Ciliary Activity Of Columnar Ciliated Epithelium Of The Nasal Mucosa In Laboratory Rats. Laboratory Animals for Science. 2018; 1. https://doi.org/10.29296/2618723X-2018-01-07

Abstract

The results of a comparative study of the intravital cytological material of the columnar ciliated epithelium of the nasal cavity from intact adult rats of the Wistar (220-250 g) contained in the conventional type vivarium (n = 2) and SPF category (n = 2) are presented. An intact picture of cells of the columnar ciliated epithelium is noted in the material of SPF animals. A subacute inflammation and persistence of bacterial infection in animals of the conventional vivarium were observed. In this case, there were morphofunctional signs of degeneration of the ciliary apparatus: shortening, asynchronism and incomplete cycle of beating of the cilia. The best quality of the cytological material for in-vivo biopsy was obtained by using the combined cervical brushes «Cervex-Brush Combi» made of a polymer material modified by trimming the bristles of the central brush.

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