Influence Of The Bedding Type On The Development Of Tactile Allodynia After Sciatic Nerve Ligation In Wistar Rats

Belozertseva I.V.(1), Kashkin V.A.(1,2), Shekunova E.V.(1,2) 1-Valdman Institute of Pharmacology, Pavlov First Saint Petersburg State Medical University, 6-8, L’va Tolstogo str., Saint Petersburg, 197022, Russia , 2 -“Institute of experimental pharmacology”, b. 245, 3, st. Zavodskaya, Kuzmolovskiy, Vsevolozhskiy district, Leningrad region, 188663, Russia, Leningrad region e-mail: belozertseva@gmail.com

Abstract

Although animal pain models may be highly sensitive to small changes in animal care, all relevant details are usually not reported in research papers, potentially resulting in increased inter-laboratory variability. The purpose of the present study was to determine whether the type of bedding rats are housed on affects the development to allodinya following sciatic nerve ligation (Chronic Constrictive Injury model - CCI). Two types of bedding were compared, the first containing spruce shavings (J.Rettenmaier&söhne GmbH+Co KG, Germany; standard bedding for our laboratory in past), and the other containing cob corn chips («KKZ «Zolotoy pochatok» LLC, Russia; standard bedding for our laboratory in present). Tactile sensitivity thresholds were assessed with von Frey filaments before and during periods of 7 or 21 days following sciatic nerve ligation in male and female Wistar rats. The results of the present experiment indicate that the bedding type that animals are housed can markedly affect the development of tactile allodynia induced by CCI. Hard cob corn bedding resulted in the least sensitive thresholds for mechanical stimuli. Therefore, careful consideration should be used when selecting routine cage bedding material for animals that will be tested in sensitivity of paw plantar surface assay.

References

  1. Bennett G. J., Xie Y. K. A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man. Pain. 1988. T. 33; 1: 87–107.
  2. Weichbrod R. H., Cisar C. F., Miller J. G., Simmonds R. C., Alvares A. P., Ueng T. H. Effects of cage beddings on microsomal oxidative enzymes in rat liver. Lab Anim Sci. 1988. T. 38; 3: 296–8.
  3. Robinson I., Dowdall T., Meert T. F. Development of neuropathic pain is affected by bedding texture in two models of peripheral nerve injury in rats. Neurosci Lett. 2004. T. 368; 1: 107–11.
  4. Brownjohn P. W., Ashton J. C. A technical note for improving animal welfare and model validity in the chronic constriction injury model of neuropathic pain. Eur J Pain. 2012. T. 16; 10: 1477.
  5. Moehring F., O’Hara C. L., Stucky C. L. Bedding Material Affects Mechanical Thresholds, Heat Thresholds, and Texture Preference. J Pain. 2016. T. 17; 1: 50–64.
  6. Belozerceva I. V. Rukovodstvo po ispol`zovaniyu laboratornyh zhivotnyh dlya nauchnyh i uchebnyh celey v PSPbGMU im. I. P. Pavlova (I. V. Belozerceva, O. A. Dravolina, M. A. Tur. Pod red. E`. E`. Zvartau). SPb. : Izd-vo SPbGMU, 2014: 79.
  7. Chaplan S. R., Bach F. W., Pogrel J. W., Chung J. M., Yaksh T. L. Quantitative assessment of tactile allodynia in the rat paw. J Neurosci Methods. 1994. T. 53; 1: 55–63.
  8. Dixon W. J. Efficient analysis of experimental observations. Annu Rev Pharmacol Toxicol. 1980. T. 20: 441–62.
  9. Motulsky H. J. Common misconceptions about data analysis and statistics. Naunyn Schmiedebergs Arch Pharmacol. 2014. T. 387; 11: 1017–23.
  10. Rowland D. L., Thornton J. A. Testing and analytical procedures for laboratory studies involving nonresponders during a limited observation period: an illustration using male sexual behavior in rats. Pharmacol Biochem Behav. 2001. T. 68; 3: 403–9.
  11. Nagel J., Belzertseva I., Greco S., Kashkin V., Malyshkin A., Jirgensons A., Shekunova E., Eilbacher B., Bespalov A., Danysz W. Effects of NAAG peptidase inhibitor 2-PMPA in model chronic pain - relation to brain concentration. Neuropharmacology. 2006. T. 51; 7–8: 1163–71.
  12. More L., Gravius A., Pietraszek M., Belozertseva I., Malyshkin A., Shekunova E., Barberi C., Schaefer D., Schmidt W. J., Danysz W. Comparison of the mGluR1 antagonist A-841720 in rat models of pain and cognition. Behav Pharmacol. 2007. T. 18; 4: 273–81.

You may be interested